{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T18:11:17Z","timestamp":1789495877671,"version":"build-2803163510"},"reference-count":45,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2008,10,1]],"date-time":"2008-10-01T00:00:00Z","timestamp":1222819200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2008,10]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> This study evaluated the safety and effectiveness of a tissue\u2010engineered skin product composed of viable neonatal keratinocytes and fibroblasts and compared it to a free gingival graft (FGG) in a procedure to enhance keratinized tissue (KT) and wound healing around teeth that do not require root coverage.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Twenty\u2010five subjects were enrolled who had at least two non\u2010adjacent teeth in contralateral quadrants exhibiting an insufficient zone of attached gingiva requiring soft tissue grafting where root coverage was not desired. One tooth was randomized to receive an FGG, and the other was randomized to receive bilayered cell therapy (BCT). The amount of KT was measured at baseline and 3 and 6 months, and the texture and color of the grafted tissue were compared to the surrounding tissue at months 1, 3, and 6. A questionnaire was used to determine subject preference at 6 months. Biopsies and persistence studies were performed on a subset of the subjects.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> The FGG generated statistically significantly (<jats:italic>P<\/jats:italic> &lt;0.001) more KT than the test device (BCT) (4.5 \u00b1 0.80 mm versus 2.4 \u00b1 1.02 mm); no significant difference in recession or clinical attachment level was detected between treatment groups (<jats:italic>P<\/jats:italic> = 0.212 and <jats:italic>P<\/jats:italic> = 0.448, respectively); and no significant differences were detected at any time point for bleeding on probing (BOP), resistance to muscle pull, or inflammation. The BCT group had significantly better color and texture match with surrounding tissue (<jats:italic>P<\/jats:italic> &lt;0.001), and subject preference was significantly greater for the BCT group (<jats:italic>P<\/jats:italic> = 0.041). No device\u2010related adverse events or safety issues occurred during the course of the study.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> The tissue\u2010engineered graft BCT was safe and capable of generating <jats:italic>de novo<\/jats:italic> KT without the morbidity and potential clinical difficulties associated with donor\u2010site surgery. The amount of KT generated with FGG was greater than generated with BCT; however, 24 of 25 test sites demonstrated an increase in KT at 6 months, with more than three\u2010quarters of the sites yielding \u22652 mm bands of KT.<\/jats:p>","DOI":"10.1902\/jop.2008.080017","type":"journal-article","created":{"date-parts":[[2008,10,3]],"date-time":"2008-10-03T09:21:16Z","timestamp":1223025676000},"page":"1847-1856","source":"Crossref","is-referenced-by-count":85,"title":["A Pilot Study to Evaluate a Tissue\u2010Engineered Bilayered Cell Therapy as an Alternative to Tissue From the Palate"],"prefix":"10.1002","volume":"79","author":[{"given":"Michael K.","family":"McGuire","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. Todd","family":"Scheyer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martha E.","family":"Nunn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip T.","family":"Lavin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,10]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1963.34.3.201"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1972.43.10.623"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1981.tb02041.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1996.1.1.671"},{"key":"e_1_2_7_6_1","first-page":"40","article-title":"Marginal tissue recession in general dental practice: A preliminary study","volume":"3","author":"Wilson R","year":"1983","journal-title":"Int J Periodontics Restorative Dent"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1979.50.4.170"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1984.tb00837.x"},{"key":"e_1_2_7_9_1","first-page":"9","article-title":"Attached gingiva \u2013 Mucogingival therapy and restorative dentistry","volume":"6","author":"Nevins M","year":"1986","journal-title":"Int J Periodontics Restorative Dent"},{"key":"e_1_2_7_10_1","first-page":"74","article-title":"The role of keratinized tissue and attached gingiva in maintaining periodontal\/peri\u2010implant health","volume":"52","author":"Marquez IC","year":"2004","journal-title":"Gen Dent"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1981.52.9.569"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1980.51.5.274"},{"key":"e_1_2_7_13_1","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1016\/S0011-8532(22)00577-8","article-title":"Periodontal plastic surgery","volume":"42","author":"McGuire MK","year":"1998","journal-title":"Dent Clin North Am"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2001.72.7.932"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibtech.2006.05.001"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1586\/17434440.3.1.29"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1055"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1054-8807(03)00089-9"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cden.2005.12.001"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0142-9612(96)85569-4"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260430809"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.240450304"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.2002.01865.x"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12539993"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.3109\/15569529309036259"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9610(03)00284-8"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1524-475X.1999.00201.x"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1001\/archsurg.135.6.627"},{"key":"e_1_2_7_29_1","first-page":"51","article-title":"A comparative study of root coverage obtained with an acellular dermal matrix versus a connective tissue graft: Results of 107 recession defects in 50 consecutively treated patients","volume":"20","author":"Harris RJ","year":"2000","journal-title":"Int J Periodontics Restorative Dent"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051X.29.s3.11.x"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1993.64.5.387"},{"key":"e_1_2_7_32_1","first-page":"127","article-title":"Comparison of rhPDGF\u2010BB + Beta TCP and a collagen membrane to subepithelial connective tissue grafting for the treatment of recession defects. A case series","volume":"26","author":"McGuire MK","year":"2006","journal-title":"Int J Periodontics Restorative Dent"},{"key":"e_1_2_7_33_1","first-page":"552","article-title":"Tissue engineering technology for gingival augmentation procedures: A case report","volume":"20","author":"Pini Prato GP","year":"2000","journal-title":"Int J Periodontics Restorative Dent"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2005.76.6.867"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2005.76.6.881"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1980.tb02011.x"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1977.48.9.587"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1972.43.1.38"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2003.74.2.262"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2002.73.7.748"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1971.tb00619.x"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1975.tb00001.x"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1973.44.12.727"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1977.48.12.768"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1981.52.2.74"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.2004.10.1180"}],"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2008.080017","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T11:52:14Z","timestamp":1760874734000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2008.080017"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,10]]},"references-count":45,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2008,10]]}},"alternative-id":["10.1902\/jop.2008.080017"],"URL":"https:\/\/doi.org\/10.1902\/jop.2008.080017","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,10]]}}}