{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T00:26:26Z","timestamp":1771028786256,"version":"3.50.1"},"reference-count":47,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2008,9,1]],"date-time":"2008-09-01T00:00:00Z","timestamp":1220227200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2008,9]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Periodontal tissues remodel rapidly, which enables quick adaptation to mechanical changes. Matrix metalloproteinases (MMPs) are involved in these remodeling processes under control of tissue inhibitor of metalloproteinases (TIMPs). In periodontitis, overactivity of MMPs results in pathologic tissue degradation. The aim of this study was to analyze MMPs and TIMPs in healthy and diseased gingiva, periodontal ligament (PDL), and gingival crevicular fluid (GCF).<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Samples of gingiva, PDL, and GCF were obtained from healthy controls (gingiva: n = 18; PDL: n = 15; GCF: n = 8) and subjects with periodontitis (gingiva: n = 11; PDL: n = 18; GCF: n = 12). MMPs and TIMPs were analyzed by gelatin\u2010, collagen\u2010, and reverse zymography and by Western blotting. Total MMP activity was analyzed using a fluorogenic substrate.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> TIMP\u20101 and \u22122, active and pro\u2010MMP\u20102 and \u22129, and active MMP\u20101 and \u22128 were present in all samples. Large amounts of active MMP\u20102 complexes and collagenolytic fragments were also found. Their levels were higher in PDL and GCF from subjects with periodontitis. In general, TIMP levels were lower in diseased periodontal tissues. Especially diseased GCF contained more MMPs. Surprisingly, some MMPs were more abundant in healthy gingiva and PDL than in diseased tissue.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> Unexpected variations in MMP and TIMP levels in gingiva, PDL, and GCF may result from differences in subject characteristics and disease activity. The levels of active MMP\u20102 complexes and collagenolytic fragments are higher in the periodontium of subjects with periodontitis and might contribute significantly to periodontal destruction.<\/jats:p>","DOI":"10.1902\/jop.2008.080021","type":"journal-article","created":{"date-parts":[[2008,9,4]],"date-time":"2008-09-04T20:03:05Z","timestamp":1220558585000},"page":"1704-1711","source":"Crossref","is-referenced-by-count":28,"title":["Collagenolytic Fragments and Active Gelatinase Complexes in Periodontitis"],"prefix":"10.1002","volume":"79","author":[{"given":"Miriam M.","family":"Bildt","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marjon","family":"Bloemen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne M.","family":"Kuijpers\u2010Jagtman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johannes W.","family":"Von 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