{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:20:46Z","timestamp":1760919646801,"version":"build-2065373602"},"reference-count":37,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2008,12,1]],"date-time":"2008-12-01T00:00:00Z","timestamp":1228089600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009068","name":"Universit\u00e4t Bern","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009068","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2008,12]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> The goal of this study was to determine whether site\u2010specific differences in the subgingival microbiota could be detected by the checkerboard method in subjects with periodontitis.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Subjects with at least six periodontal pockets with a probing depth (PD) between 5 and 7 mm were enrolled in the study. Subgingival plaque samples were collected with sterile curets by a single\u2010stroke procedure at six selected periodontal sites from 161 subjects (966 subgingival sites). Subgingival bacterial samples were assayed with the checkerboard DNA\u2010DNA hybridization method identifying 37 species.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Probing depths of 5, 6, and 7 mm were found at 50% (n = 483), 34% (n = 328), and 16% (n = 155) of sites, respectively. Statistical analysis failed to demonstrate differences in the sum of bacterial counts by tooth type (<jats:italic>P<\/jats:italic> = 0.18) or specific location of the sample (<jats:italic>P<\/jats:italic> = 0.78). With the exceptions of <jats:italic>Campylobacter gracilis<\/jats:italic> (<jats:italic>P<\/jats:italic> &lt;0.001) and <jats:italic>Actinomyces naeslundii<\/jats:italic> (<jats:italic>P<\/jats:italic> &lt;0.001), analysis by general linear model multivariate regression failed to identify subject or sample location factors as explanatory to microbiologic results. A trend of difference in bacterial load by tooth type was found for <jats:italic>Prevotella nigrescens<\/jats:italic> (<jats:italic>P<\/jats:italic> &lt;0.01). At a cutoff level of \u22651.0 \u00d7 10<jats:sup>5<\/jats:sup>, <jats:italic>Porphyromonas gingivalis<\/jats:italic> and <jats:italic>Tannerella forsythia<\/jats:italic> (previously <jats:italic>T. forsythensis<\/jats:italic>) were present at 48.0% to 56.3% and 46.0% to 51.2% of sampled sites, respectively.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> Given the similarities in the clinical evidence of periodontitis, the presence and levels of 37 species commonly studied in periodontitis are similar, with no differences between molar, premolar, and incisor\/cuspid subgingival sites. This may facilitate microbiologic sampling strategies in subjects during periodontal therapy.<\/jats:p>","DOI":"10.1902\/jop.2008.080142","type":"journal-article","created":{"date-parts":[[2008,12,4]],"date-time":"2008-12-04T07:12:25Z","timestamp":1228374745000},"page":"2290-2296","source":"Crossref","is-referenced-by-count":16,"title":["Similarities in the Subgingival Microbiota Assessed by a Curet Sampling Method at Sites With Chronic Periodontitis"],"prefix":"10.1002","volume":"79","author":[{"given":"G. 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