{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T01:14:13Z","timestamp":1790990053941,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2008,12,1]],"date-time":"2008-12-01T00:00:00Z","timestamp":1228089600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2008,12]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> The purpose of this study was to test the hypothesis that periodontal pathogens associated with aggressive periodontitis persist in extracrevicular locations following scaling and root planing, systemic antibiotics, and antimicrobial rinses.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Eighteen patients with aggressive periodontitis received a clinical examination during which samples of subgingival plaque and buccal epithelial cells were obtained. Treatment consisted of full\u2010mouth root planing, systemic antibiotics, and chlorhexidine rinses. Clinical measurements and sampling were repeated at 3 and 6 months. Quantitative polymerase chain reaction determined the number of <jats:italic>Aggregatibacter actinomycetemcomitans<\/jats:italic> (previously <jats:italic>Actinobacillus actinomycetemcomitans<\/jats:italic>), <jats:italic>Prevotella intermedia<\/jats:italic>, <jats:italic>Porphyromonas gingivalis<\/jats:italic>, <jats:italic>Tannerella forsythia<\/jats:italic> (previously <jats:italic>T. forsythensis<\/jats:italic>), and <jats:italic>Treponema denticola<\/jats:italic> in the plaque. Fluorescence in situ hybridization and confocal microscopy determined the extent of intracellular invasion in epithelial cells.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Clinical measurements improved significantly following treatment. All bacterial species except <jats:italic>P. gingivalis<\/jats:italic> were significantly reduced in plaque between baseline and 3 months. However, all species showed a trend to repopulate between 3 and 6 months. This increase was statistically significant for log <jats:italic>T. denticola<\/jats:italic> counts. All species were detected intracellularly. The percentage of cells infected intracellularly was not affected by therapy.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> The 6\u2010month increasing trend in the levels of plaque bacteria suggests that subgingival recolonization was occurring. Because the presence of these species within epithelial cells was not altered after treatment, it is plausible that recolonization may occur from the oral mucosa. Systemic antibiotics and topical chlorhexidine did not reduce the percentage of invaded epithelial cells. These data support the hypothesis that extracrevicular reservoirs of bacteria exist, which might contribute to recurrent or refractory disease in some patients.<\/jats:p>","DOI":"10.1902\/jop.2008.080254","type":"journal-article","created":{"date-parts":[[2008,12,4]],"date-time":"2008-12-04T07:12:25Z","timestamp":1228374745000},"page":"2305-2312","source":"Crossref","is-referenced-by-count":78,"title":["Persistence of Extracrevicular Bacterial Reservoirs After Treatment of Aggressive Periodontitis"],"prefix":"10.1002","volume":"79","author":[{"given":"Jason D.","family":"Johnson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruoqiong","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia 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