{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T09:40:14Z","timestamp":1760866814410,"version":"build-2065373602"},"reference-count":45,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2009,4,1]],"date-time":"2009-04-01T00:00:00Z","timestamp":1238544000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2009,4]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> A genome\u2010association study is a powerful tool for analyzing small gene effects in complex diseases such as chronic periodontitis (CP), although the cost of analysis is prohibitive. We designed a study using the DNA pooling method, which could be a breakthrough in lowering such costs. This study was conducted to assess the genetic association in severe CP in a Japanese population.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> We adopted a DNA pooling method by genotyping 454 densely spaced microsatellite (MS) markers in chromosome 19 as a pilot study, with the possibility of future use in a whole\u2010genome study. This can reduce the high cost and technical burden, which is generally unavoidable in a genomic association study. Pooled DNA samples from 300 case subjects, 300 control subjects, and 200 systemically healthy subjects were screened by genotyping MS markers. The case\u2010control association in the candidate region was analyzed by individual typing of MS and single nucleotide polymorphisms (SNPs).<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> The single MS marker allele 17 of 1902G31 was isolated in association with severe CP (<jats:italic>P =<\/jats:italic> 0.0012 for 2 \u00d7 2; <jats:italic>P &lt;<\/jats:italic>0.046 for 2 \u00d7 m, where m refers to the number of polymorphic alleles observed in a population). No other SNP or MS polymorphism hypothesized to affect biologic functions in the critical region was found in the linkage disequilibrium block analysis.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> We efficiently isolated the susceptible locus for severe CP in chromosome 19 and identified a useful marker to evaluate the risk for disease. This approach can be applied to a whole\u2010genome study in severe CP.<\/jats:p>","DOI":"10.1902\/jop.2009.080516","type":"journal-article","created":{"date-parts":[[2009,3,31]],"date-time":"2009-03-31T09:42:01Z","timestamp":1238492521000},"page":"663-671","source":"Crossref","is-referenced-by-count":4,"title":["Assessment of Chromosome 19 for Genetic Association in Severe Chronic Periodontitis"],"prefix":"10.1002","volume":"80","author":[{"given":"Koichi","family":"Tabeta","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuko","family":"Shimada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideaki","family":"Tai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuichi","family":"Ishihara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshihide","family":"Noguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihiko","family":"Soga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shogo","family":"Takashiba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Genki","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Terukazu","family":"Kobayashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Oka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuo","family":"Kobayashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhisa","family":"Yamazaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidetoshi","family":"Inoko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiromasa","family":"Yoshie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,4]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1991.tb01649.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1998.tb02454.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910308200808"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1994.65.5s.479"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1997.tb01187.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1999.70.4.418"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2001.281208.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.2006.00164.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1999.tb02270.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051x.2000.027009682.x"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2001.72.11.1554"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051x.2001.028005430.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051x.2002.290105.x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.273.5281.1516"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1166"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/8.12.2165"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1086\/302364"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1086\/302786"},{"key":"e_1_2_7_20_1","first-page":"3702","article-title":"Localization of the pathogenic gene of Behcet's disease by microsatellite analysis of three different populations","volume":"41","author":"Mizuki N","year":"2000","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2008.03.016"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.0000257256.77680.02"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddi234"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2005050493"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2003.08.020"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1999.4.1.1"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-0039.2003.00007.x"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004390051031"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.229102"},{"key":"e_1_2_7_30_1","unstructured":"Sputnik.DNA microsatellite repeat search utility.Available at: http:\/\/espressosoftware.com\/pages\/sputnik.jsp. Accessed January 22 2007."},{"key":"e_1_2_7_31_1","unstructured":"NCBI.Single nucleotide polymorphism.Available at http:\/\/www.ncbi.nlm.nih.gov\/projects\/SNP\/. Accessed September 27 2007."},{"key":"e_1_2_7_32_1","doi-asserted-by":"crossref","unstructured":"International HapMap project. HapMap genome browser.Available at: http:\/\/www.hapmap.org\/. Accessed September 27 2007.","DOI":"10.69645\/ZEQN7784"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jhered.a111573"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1086\/502802"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1086\/505539"},{"issue":"3","key":"e_1_2_7_36_1","first-page":"III40","article-title":"Toward identification of susceptibility genes for sporadic Parkinson's disease","volume":"250","author":"Toda T","year":"2003","journal-title":"J Neurol"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bth457"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/18.6.1595"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.tpj.6500132"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ahj.2005.03.011"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051X.2002.290409.x"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2006.050315"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0001-2815.2004.0228.x"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910708600505"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gene.6363900"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1999.70.10.1194"}],"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2009.080516","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T09:19:43Z","timestamp":1760865583000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2009.080516"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,4]]},"references-count":45,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2009,4]]}},"alternative-id":["10.1902\/jop.2009.080516"],"URL":"https:\/\/doi.org\/10.1902\/jop.2009.080516","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"type":"print","value":"0022-3492"},{"type":"electronic","value":"1943-3670"}],"subject":[],"published":{"date-parts":[[2009,4]]}}}