{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T03:14:15Z","timestamp":1789528455063,"version":"build-2803163510"},"reference-count":19,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2009,7,1]],"date-time":"2009-07-01T00:00:00Z","timestamp":1246406400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2009,7]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Although previous studies revealed the presence of autoreactive B cells (B\u20101a cells) in periodontitis lesions, no evidence was provided for an active role of such cells in the host response to microbial challenge. The aim of the present investigation was to study the reaction of B\u20101a cells to <jats:italic>de novo<\/jats:italic> plaque formation in subjects who were treated for severe chronic periodontitis.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Fifteen white subjects with generalized, severe chronic periodontitis volunteered. Surgical periodontal therapy was performed in all quadrants of each subject after a period of infection control. After 6 months of healing (baseline), two gingival biopsies were harvested from each patient (probing depth &lt;4 mm and no bleeding on probing; healed sites). The experimental gingivitis model was applied, and plaque accumulation was allowed for 3 weeks. Two additional biopsies were collected and prepared for immunohistochemical analysis on day 21.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> The biopsies retrieved after 3 weeks of plaque accumulation contained larger proportions of CD19+ and CD5+ cells (B\u20101a cells) than biopsies representing baseline (healed sites) (7.38% \u00b1 2.80% versus 5.96% \u00b1 2.48%). The tissue fraction of cells carrying the markers for CD3 (T cells), CD19 (B cells), and Bcl2 (apoptosis\u2010associated marker) were significantly larger in tissue samples collected after 3 weeks of plaque accumulation than in specimens from baseline (healed sites).<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> Autoreactive B cells (B\u20101a cells) are involved in the host response to microbial challenge in subjects with chronic periodontitis.<\/jats:p>","DOI":"10.1902\/jop.2009.080660","type":"journal-article","created":{"date-parts":[[2009,6,30]],"date-time":"2009-06-30T07:05:18Z","timestamp":1246345518000},"page":"1141-1145","source":"Crossref","is-referenced-by-count":8,"title":["B\u20101a Cells in Experimental Gingivitis in Humans"],"prefix":"10.1002","volume":"80","author":[{"given":"Mauro","family":"Donati","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birgitta","family":"Liljenberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola U.","family":"Zitzmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tord","family":"Berglundh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2005.00820.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2008.01178.x"},{"key":"e_1_2_7_4_1","first-page":"281","article-title":"Autoimmunity and periodontal disease","volume":"73","author":"Brandtzaeg P","year":"1965","journal-title":"Odontol Tidskr"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1986.57.11.693"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1992.tb01814.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.1988.tb01315.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1991.tb00113.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(91)90136-H"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1992.tb01822.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1023234823783"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051X.2002.290402.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2005.00649.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1177\/29.4.6166661"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2008.070299"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1986.tb00846.x"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051X.1999.260707.x"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051x.2001.028006517.x"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2001.72.4.517"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/1746-160X-2-17"}],"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2009.080660","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T12:25:15Z","timestamp":1760876715000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2009.080660"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,7]]},"references-count":19,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2009,7]]}},"alternative-id":["10.1902\/jop.2009.080660"],"URL":"https:\/\/doi.org\/10.1902\/jop.2009.080660","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,7]]}}}