{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T13:32:25Z","timestamp":1791379945629,"version":"4.3.3"},"reference-count":50,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2010,1]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Diabetes and periodontal diseases are often associated. Both have highly inflammatory components, but the role played by distinct phlogistic mediators in their pathogenesis is not fully understood and remains controversial. The purpose of this study is to evaluate whether type 2 diabetes alters the expression of inflammatory mediators in sites with chronic periodontitis (CP) or peri\u2010implantitis (P\u2010IM).<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> The expression of tumor necrosis factor (TNF)\u2010\u03b1, interleukin (IL)\u20106 and \u20108, and monocyte chemotactic protein (MCP)\u20101 plus key CC chemokine receptors (CCR1 through 5) and CXC chemokine receptors (CXCR1 through 3) was quantified by real\u2010time polymerase chain reaction (PCR) in gingival or peri\u2010implant biopsies from 135 patients with well\u2010controlled or poorly controlled diabetes and periodontal disease, 65 patients with periodontal disease but otherwise healthy, and 90 systematically and periodontally healthy subjects. Western blots were performed.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Relative to controls, in patients without diabetes and patients with well\u2010controlled diabetes, TNF\u2010\u03b1, CCR5, and CXCR3 expression was exclusively higher in sites with P\u2010IM (<jats:italic>P<\/jats:italic> &lt;0.01), whereas IL\u20106 and \u20108 were overexpressed in sites with CP and, even more, in sites with P\u2010IM (<jats:italic>P<\/jats:italic> &lt;0.01). In patients with poor glycemic control, TNF\u2010\u03b1, CCR5, and CXCR3 mRNAs were increased in sites with CP (<jats:italic>P<\/jats:italic> &lt;0.01). A statistically significant higher IL\u20106 and \u20108 expression from patients without diabetes and patients with well\u2010controlled diabetes was observed compared to patients with poorly controlled diabetes. Regardless of metabolic\/glycemic status, MCP\u20101 and CCR2 and 4 were markedly higher in both of the oral pathologies examined (<jats:italic>P<\/jats:italic> &lt;0.01). At the protein levels, Western blot experiments confirmed the real\u2010time PCR results.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> These findings showed that: 1) in subjects without diabetes and patients with well\u2010controlled diabetes, TNF\u2010\u03b1, CCR5, and CXCR3 may constitute distinctive biomarkers of P\u2010IM; 2) poor glycemic control abolished the differences between CP and P\u2010IM regarding the expression of these mediators; and 3) type 2 diabetes affected the expression of TNF\u2010\u03b1, IL\u20106 and \u20108, CCR5, and CXCR3.<\/jats:p>","DOI":"10.1902\/jop.2009.090358","type":"journal-article","created":{"date-parts":[[2010,1,11]],"date-time":"2010-01-11T03:56:32Z","timestamp":1263182192000},"page":"99-108","source":"Crossref","is-referenced-by-count":100,"title":["Proinflammatory Gene Expression at Chronic Periodontitis and Peri\u2010Implantitis Sites in Patients With or Without Type 2 Diabetes"],"prefix":"10.1002","volume":"81","author":[{"given":"Isabella","family":"Venza","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Visalli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Cucinotta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppina","family":"De Grazia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Teti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Venza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,1]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.2001.6.1.125"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1998.3.1.51"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.2337\/diaclin.23.4.171"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.14219\/jada.archive.2006.0404"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.14219\/jada.archive.2003.0370"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1998.3.1.40"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1998.3.1.20"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2006.050459"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910608500103"},{"key":"e_1_2_7_11_1","first-page":"1","article-title":"Periodontal diseases in diabetics: Relationship, prevention, and treatment","volume":"1","author":"Schulze A","year":"2008","journal-title":"CSMI"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2006.00984.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2008.04.013"},{"key":"e_1_2_7_14_1","first-page":"485","article-title":"Relationship between neutrophil functions and severity of periodontitis in obese and\/or type 2 diabetic chronic periodontitis patients","volume":"39","author":"Gursoy UK","year":"2008","journal-title":"Quintessence Int"},{"key":"e_1_2_7_15_1","first-page":"16","article-title":"Periodontal disease as a part of the insulin resistance syndrome in diabetic patients","volume":"7","author":"Nishimura F","year":"2005","journal-title":"J Int Acad Periodontol"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2006.050373"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2008.01082.x"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2006.01348.x"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2006.00961.x"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.87.8.1038"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.1987.tb00298.x"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1989.tb00863.x"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1999.4.1.1"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.1998.tb00255.x"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0501.2008.01680.x"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2007.01190.x"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2008.01491.x"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910808700311"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2005.76.12.2276"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1607-551X(09)70484-X"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2004.12.018"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2009.080480"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0501.2002.130610.x"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1990.tb00906.x"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1834-7819.2008.01083.x"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2005.76.11.1849"},{"key":"e_1_2_7_37_1","first-page":"135","article-title":"Immunohistochemical evaluation of inflammatory mediators in failing implants","volume":"26","author":"Konttinen YT","year":"2006","journal-title":"Int J Periodontics Restorative Dent"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1998.3.1.13"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI8942"},{"key":"e_1_2_7_40_1","first-page":"1018","article-title":"Diabetes mellitus and periodontal disease \u2013 A current perspective","volume":"15","author":"Shlossman M","year":"1994","journal-title":"Compendium"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.6.3463"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1999.70.11.1313"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.2334\/josnusd.41.163"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1986.tb00885.x"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1995.66.10.852"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2000.71.3.460"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0765.2003.02012.x"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.2003.02070.x"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.148.3.760"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1086\/515178"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-007-0148-6"}],"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2009.090358","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T11:43:48Z","timestamp":1760874228000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2009.090358"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,1]]},"references-count":50,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2010,1]]}},"alternative-id":["10.1902\/jop.2009.090358"],"URL":"https:\/\/doi.org\/10.1902\/jop.2009.090358","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,1]]}}}