{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T14:26:03Z","timestamp":1792506363092,"version":"4.5.0"},"reference-count":25,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2010,7,1]],"date-time":"2010-07-01T00:00:00Z","timestamp":1277942400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005609","name":"Turun Yliopisto","doi-asserted-by":"publisher","award":["388\/211\/2009"],"award-info":[{"award-number":["388\/211\/2009"]}],"id":[{"id":"10.13039\/501100005609","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2010,7]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> The present study evaluates the survival capability of <jats:italic>Fusobacterium nucleatum<\/jats:italic> strains in an aerobic environment and compares the invasive capability of <jats:italic>F. nucleatum<\/jats:italic> in biofilm and planktonic forms in an organotypic cell culture (OCC) model.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Biofilms of <jats:italic>F. nucleatum<\/jats:italic> American Type Culture Collection (ATCC) 25586 or Anaerobe Helsinki Negative (AHN) 9508 were produced by culturing on semipermeable membranes on brucella agar plates. The oxygen tolerance of the <jats:italic>F. nucleatum<\/jats:italic> strains was examined by incubating 3\u2010day\u2010old anaerobically grown biofilms in an aerobic environment (CO<jats:sub>2<\/jats:sub> [5% in air] incubator) for an additional 48 hours. The OCC model was constructed by seeding keratinocytes on a fibroblast\u2010containing collagen gel. In invasion assays, a 3\u2010day\u2010old anaerobically grown biofilm (and planktonic bacteria in solution as the control) was placed upside down on the top of OCC and incubated under 5% CO<jats:sub>2<\/jats:sub> for 24 hours. Invasion of the bacteria and morphologic changes in OCC were assessed using hematoxylin and eosin, Ki\u201067, and periodic acid\u2010Schiff stainings.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> In biofilms, both <jats:italic>F. nucleatum<\/jats:italic> strains continuously increased their cell numbers in an aerobic environment for 48 hours. After incubating the bacterial biofilm in contact with the OCC model, <jats:italic>F. nucleatum<\/jats:italic> AHN 9508 was able to pass through the epithelial\/basement membrane barrier and invade the collagen matrix. The invasiveness of biofilm <jats:italic>F. nucleatum<\/jats:italic> ATCC 25586 was limited to the epithelium. Cytotoxic effects and invasiveness of <jats:italic>F. nucleatum<\/jats:italic> on the OCC were much stronger when the bacteria were in biofilms than in the planktonic form.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> Biofilm formation regulates the survival and invasiveness of <jats:italic>F. nucleatum<\/jats:italic> in an aerobic environment.<\/jats:p>","DOI":"10.1902\/jop.2010.090664","type":"journal-article","created":{"date-parts":[[2010,3,29]],"date-time":"2010-03-29T12:39:13Z","timestamp":1269866353000},"page":"1084-1091","source":"Crossref","is-referenced-by-count":77,"title":["Biofilm Formation Enhances the Oxygen Tolerance and Invasiveness of <i>Fusobacterium nucleatum<\/i> in an Oral Mucosa Culture Model"],"prefix":"10.1002","volume":"81","author":[{"given":"Ulvi Kahraman","family":"Gursoy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marja","family":"P\u00f6ll\u00e4nen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eija","family":"K\u00f6n\u00f6nen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veli\u2010Jukka","family":"Uitto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-39-4-246"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.2005.00107.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.175.11.3247-3252.1993"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.2.1171-1179.2005"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0463.2008.00868.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.2008.00453.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1006\/anae.2001.0392"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.10.4729-4732.1998"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03401.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2005.00790.x"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-9-18"},{"key":"e_1_2_7_13_1","first-page":"1.7","article-title":"Growing and analyzing static biofilms","volume":"1","author":"Merritt JH","year":"2005","journal-title":"Curr Protoc Microbiol"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.70.9.6524-6528.1996"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2005.07.002"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.5.1485-1491.2003"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1838"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46417-0"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2006.05.007"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00041-08"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/424134a"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0407460101"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004030050461"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00403-003-0419-2"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-1811(01)00135-9"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.1997.tb00004.x"}],"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2010.090664","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T11:22:56Z","timestamp":1760872976000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2010.090664"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,7]]},"references-count":25,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2010,7]]}},"alternative-id":["10.1902\/jop.2010.090664"],"URL":"https:\/\/doi.org\/10.1902\/jop.2010.090664","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,7]]}}}