{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T00:26:37Z","timestamp":1792715197100,"version":"4.5.0"},"reference-count":37,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2010,11,1]],"date-time":"2010-11-01T00:00:00Z","timestamp":1288569600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2010,11]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Hydrogen sulfide (H<jats:sub>2<\/jats:sub>S), the main component of halitosis, is one of the etiologic factors for periodontitis. We recently reported that H<jats:sub>2<\/jats:sub>S may induce pathologic changes in rat alveolar bone. The objective of this study is to determine the effect of H<jats:sub>2<\/jats:sub>S on osteoclast differentiation.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Murine macrophage cells RAW264 were cultured in medium lacking nuclear factor \u03baB ligand (receptor activator of nuclear factor \u03baB ligand) in 5% CO<jats:sub>2<\/jats:sub> with air at 37\u00b0C for 24 hours; then 0.05, 0.5, or 5 ng\/ml H<jats:sub>2<\/jats:sub>S was added to the CO<jats:sub>2<\/jats:sub>\u2010air mix for 4 days. The controls received the CO<jats:sub>2<\/jats:sub>\u2010air mix with no H<jats:sub>2<\/jats:sub>S. Cell differentiation was evaluated by counting the tartrate\u2010resistant acid\u2010phosphatase (TRAP)\u2013positive cells. Extracellular signaling\u2010regulated kinase1\/2 (ERK1\/2) and mitogen\u2010activated protein kinase p38 phosphorylation were examined by Western blotting. The bone\u2010resorption activity was determined with the resorption assay of calcium phosphate.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> There were significantly more TRAP\u2010positive cells at a concentration of 0.05 ng\/ml H<jats:sub>2<\/jats:sub>S than at the other concentrations (<jats:italic>P<\/jats:italic> &lt;0.001). Cathepsin K protein, a specific marker for osteoclasts, was expressed in the H<jats:sub>2<\/jats:sub>S\u2010induced multinuclear cells. Resorption of calcium phosphate significantly increased in the H<jats:sub>2<\/jats:sub>S\u2010induced TRAP\u2010positive cells cultured on plates coated with calcium phosphate apatite (<jats:italic>P<\/jats:italic> &lt;0.01). The phosphorylation of ERK1\/2 and p38 were accelerated by H<jats:sub>2<\/jats:sub>S, and increased with time. PD98059 and SB203580, specific inhibitors of ERK1\/2 and p38, suppressed the activation of these enzymes and osteoclast differentiation by H<jats:sub>2<\/jats:sub>S.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> Results demonstrate that H<jats:sub>2<\/jats:sub>S at physiologic concentrations in mouth air induces osteoclasts from RAW264 cells.<\/jats:p>","DOI":"10.1902\/jop.2010.100116","type":"journal-article","created":{"date-parts":[[2010,6,28]],"date-time":"2010-06-28T14:26:10Z","timestamp":1277735170000},"page":"1691-1697","source":"Crossref","is-referenced-by-count":27,"title":["Oral Malodorous Compound Induces Osteoclast Differentiation Without Receptor Activator of Nuclear Factor \u03baB Ligand"],"prefix":"10.1002","volume":"81","author":[{"given":"Hisataka","family":"Ii","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshio","family":"Imai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ken","family":"Yaegaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koichiro","family":"Irie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisuke","family":"Ekuni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manabu","family":"Morita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1992.tb01673.x"},{"key":"e_1_2_7_3_1","first-page":"257","article-title":"Examination, classification, and treatment of halitosis: Clinical perspectives","volume":"66","author":"Yaegaki K","year":"2000","journal-title":"J Can Dent Assoc"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.1992.tb00641.x"},{"key":"e_1_2_7_5_1","first-page":"233","article-title":"The possible role of hydrogen sulfide in human periodontal disease. 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