{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T22:55:01Z","timestamp":1782860101644,"version":"3.54.5"},"reference-count":39,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2010,11,1]],"date-time":"2010-11-01T00:00:00Z","timestamp":1288569600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2010,11]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Matrix metalloproteinase (MMP)\u20108 is a central mediator in chronic periodontitis. MMP\u20108 can be activated by the cooperative action of other MMPs such as MMP\u201014, reactive oxygen species, and microbial proteases. The aim of this study is to associate the levels, molecular forms, isoenzyme distribution, and degree of activation of MMP\u20108 and \u201014, myeloperoxidase (MPO), and tissue inhibitor of MMP (TIMP)\u20101 in gingival crevicular fluid (GCF) from patients with progressive periodontitis at baseline and after periodontal therapy.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> In this longitudinal study, GCF samples from active (n = 25) and inactive (n = 25) sites of subjects with periodontitis were screened at baseline for GCF levels of MMP\u20108 by immunofluorometric assay, of MMP\u201014 by specific activity assay, and of MPO and TIMP\u20101 by enzyme\u2010linked immunosorbent assay. MMP\u20108 and MPO were also measured after periodontal treatment. Molecular forms were determined by immuno\u2010Western blot analyses and subjected to densitometric scanning and statistical analyses.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> High MMP\u20108 and MPO levels and a strong MPO\/MMP\u20108 positive correlation were found in active and inactive sites at baseline. After treatment, decreases in MPO and MMP\u20108 were seen, except for active sites in which MMP\u20108 differences were not significant (<jats:italic>P<\/jats:italic> &lt;0.05).<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> We present initial data that show that GCF levels and associations between MPO and MMP\u20108 are related to progression episodes and treatment responses in patients with chronic periodontitis. Our results suggest an interaction between the MPO oxidative pathway and MMP\u20108 activation, and this cascade might be useful as a potential biomarker for treatment outcomes.<\/jats:p>","DOI":"10.1902\/jop.2010.100196","type":"journal-article","created":{"date-parts":[[2010,7,23]],"date-time":"2010-07-23T14:56:08Z","timestamp":1279896968000},"page":"1644-1652","source":"Crossref","is-referenced-by-count":91,"title":["Associations Between Matrix Metalloproteinase\u20108 and \u201014 and Myeloperoxidase in Gingival Crevicular Fluid From Subjects With Progressive Chronic Periodontitis: A Longitudinal 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