{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:47:35Z","timestamp":1790729255131,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2011,4,1]],"date-time":"2011-04-01T00:00:00Z","timestamp":1301616000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2011,4]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Enterococci are often associated with chronic and recurrent infectious diseases because of their antimicrobial resistance. The aim of this study is to assess antimicrobial susceptibility of <jats:italic>Enterococcus faecalis<\/jats:italic> in chronic periodontitis.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Antimicrobial susceptibility was determined on 23 <jats:italic>E. faecalis<\/jats:italic> strains isolated from patients with chronic periodontitis. Ampicillin, erythromycin, gentamicin, tetracycline, triclosan, and vancomycin were prepared in two\u2010fold serial dilution up to 8,192 \u03bcg\/mL. Enterococcal biofilm was established by a biofilm device and observed by confocal laser microscopy and scanning electron microscopy. The minimum inhibitory concentration (MIC), minimum biofilm inhibitory concentration, and minimum biofilm eradication concentration were determined by spectrophotometer at optical density<jats:sub>650<\/jats:sub>.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> A few patches of monolayer early biofilm were observed on the surfaces of biofilm device pegs. The colony\u2010forming units of biofilm per peg were 1.2 \u00d7 10<jats:sup>3<\/jats:sup> to 1.7 \u00d7 10<jats:sup>4<\/jats:sup> and 0 to 20 post\u2010triclosan treatment. The MIC<jats:sub>50<\/jats:sub> was higher than the MIC epidemiologic cut\u2010off for tetracycline and the MIC<jats:sub>90<\/jats:sub> was higher than the cut\u2010off for erythromycin and tetracycline, respectively. In biofilm, minimum biofilm eradication concentrations were extremely high for all of the drugs except triclosan.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> The <jats:italic>E. faecalis<\/jats:italic> strains of chronic periodontitis exhibited weak biofilm formation ability at the early stage. Over 50% of the strains were resistant to tetracycline, and a few strains were highly resistant to erythromycin or gentamicin. <jats:italic>E. faecalis<\/jats:italic> cells in biofilm were hardly eradicated by most of the agents, even in high concentrations. Triclosan was effective in inhibiting <jats:italic>E. faecalis<\/jats:italic> growth in both biofilm and planktonic phase.<\/jats:p>","DOI":"10.1902\/jop.2010.100378","type":"journal-article","created":{"date-parts":[[2010,11,8]],"date-time":"2010-11-08T05:23:42Z","timestamp":1289193822000},"page":"626-631","source":"Crossref","is-referenced-by-count":19,"title":["Assessment of Antimicrobial Susceptibility of <i>Enterococcus Faecalis<\/i> Isolated From Chronic Periodontitis in Biofilm Versus Planktonic Phase"],"prefix":"10.1002","volume":"82","author":[{"given":"Jinglu","family":"Sun","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaobo","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,4]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1086\/591861"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.7.4.462"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01963632"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2007.00836.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.10.4538-4545.2001"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.47331-0"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.05201-0"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-1546.2003.00054.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0722.2004.00165.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004770-200210000-00003"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2591.2004.00865.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-302x.2000.150507.x"},{"key":"e_1_2_7_14_1","first-page":"420","volume-title":"Clinical Periodontology and Implant Dentistry","author":"Kinane DF","year":"2008"},{"key":"e_1_2_7_15_1","first-page":"103","volume-title":"Oral Microbiology","author":"Marsh PD","year":"2009"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.4.1443-1446.2003"},{"key":"e_1_2_7_17_1","first-page":"594","article-title":"The periodontal disease classification system of the American Academy of Periodontology \u2014 An update","volume":"66","author":"Wiebe CB","year":"2000","journal-title":"J Can Dent Assoc"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1998.tb02424.x"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1995.tb00166.x"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.4317\/medoral.15.e667"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2007.08.004"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.1992.tb00034.x"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00388-09"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1251\/bpo127"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/48.suppl_1.5"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0450.2006.00962.x"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.6.1771-1776.1999"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.10.6032-6039.2004"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.1.154-163.2004"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.17.5629-5639.2004"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.6.3658-3663.2004"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1086\/423139"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00430-001-0096-8"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfm141"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.5.1915-1922.2004"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00430-002-0125-2"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.blo.0000175714.68624.74"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1067\/mic.2000.102378"},{"key":"e_1_2_7_39_1","first-page":"40640","article-title":"Over\u2010the\u2010counter drug products; 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