{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:27:20Z","timestamp":1791268040005,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2011,5,1]],"date-time":"2011-05-01T00:00:00Z","timestamp":1304208000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2011,5]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Blood\u2010derived products, platelet\u2010poor plasma (PPP) and platelet\u2010rich plasma (PRP), constitute an approach in the enhancement of tissue healing. PRP has also been used as a scaffold for bone marrow stem cells in tissue engineering. This study evaluates the effect of PPP, calcium chloride\u2013activated PRP (PRP\/Ca), calcium chloride\u2013 and thrombin\u2010activated PRP (PRP\/Thr\/Ca), and bone marrow mononuclear cells and PRP\/Ca (BMMCs\/PRP\/Ca) on the healing of replanted dog teeth.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> After 30 minutes of extraction, teeth were replanted with 1) no material (control); 2) PPP; 3) PRP\/Ca; 4) PRP\/Thr\/Ca; or 5) BMMCs\/PRP\/Ca. Histologic, histomorphometric, and immunohistochemical analysis was assessed 120 days after replantation. Data from histomorphometric analysis were analyzed statistically (analysis of variance, Tukey; <jats:italic>P<\/jats:italic> &lt;0.05). Quantitative immunohistochemical analysis was analyzed by Kruskal\u2010Wallis and Dunn post hoc test (<jats:italic>P<\/jats:italic> &lt;0.05).<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Flow cytometry analysis showed 55.98% of CD34<jats:sup>+<\/jats:sup> and 32.67% of CD90\/Thy\u20101 for BMMCs sample. BMMCs\/PRP\/Ca presented the largest areas of replacement resorption characterized by osseous ingrowth into cementum (<jats:italic>P<\/jats:italic> &lt;0.05), with intense immunomarcation for tartrate\u2010resistant acid phosphatase. The PRP\/Ca group also showed areas of replacement resorption with significant immunomarcation for osteopontin. PRP\/Thr\/Ca presented no replacement resorption. PPP showed areas of inflammatory resorption, with immunomarcation for tartrate\u2010resistant acid phosphatase.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> The results suggest that platelets activated with thrombin play an important role in the healing of tissues after tooth replantation. Additional studies are necessary to test other materials, because PRP\/Ca did not present an appropriate scaffold for undifferentiated cells in the treatment of avulsed teeth.<\/jats:p>","DOI":"10.1902\/jop.2010.100448","type":"journal-article","created":{"date-parts":[[2010,11,8]],"date-time":"2010-11-08T05:23:42Z","timestamp":1289193822000},"page":"758-766","source":"Crossref","is-referenced-by-count":20,"title":["Periodontal Tissue Engineering After Tooth Replantation"],"prefix":"10.1002","volume":"82","author":[{"given":"Luciana","family":"Reichert da Silva Assun\u00e7\u00e3o","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renato","family":"Colenci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caril Constante","family":"Ferreira do\u2010Amaral","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celso Koogi","family":"Sonoda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suely Regina","family":"Mogami Bomfim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta","family":"Okamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marjorie","family":"de Assis Golim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elenice","family":"Deffune","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9lio","family":"Percinoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra Helena","family":"Penha de Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,5]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"383","volume-title":"Textbook and Color Atlas of Traumatic Injuries to the Teeth","author":"Andreasen JO","year":"1994"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-9657.2005.00316.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345550340041301"},{"key":"e_1_2_7_5_1","first-page":"423","article-title":"Root surface treatment with vitamin C in tooth replantation: Microscopic study in rats","volume":"57","author":"Panzarini SR","year":"2008","journal-title":"Minerva Stomatol"},{"key":"e_1_2_7_6_1","first-page":"13","volume-title":"Textbook and Color Atlas of Traumatic Injuries to the Teeth","author":"Gottrup F","year":"1994"},{"key":"e_1_2_7_7_1","first-page":"673","article-title":"Fibrin containing gels induce angiogenesis. 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