{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T16:38:09Z","timestamp":1777480689439,"version":"3.51.4"},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,1,1]],"date-time":"2012-01-01T00:00:00Z","timestamp":1325376000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> The effect of advanced glycation end products (AGEs) on gingival inflammation has not been fully elucidated. This study aims to investigate the hypothesis that AGEs may enhance the expression of matrix metalloproteinase\u20101 (MMP\u20101) of human gingival fibroblasts (HGFs) and to explore whether the signal pathway receptor for AGE (RAGE)\/nuclear factor\u2010\u03baB (NF\u2010\u03baB) are involved in the expression of MMP\u20101 in HGFs.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Cultured HGFs from 12 healthy gingival human tissue samples were coincubated with AGEs for the detection of MMP\u20101 protein and mRNA. Thirty\u2010six gingival samples were collected and treated for the determination of RAGE, NF\u2010\u03baB, and MMP\u20101 mRNA level in gingival connective tissues from the participants with chronic periodontitis, diabetes\u2010associated periodontitis, and healthy controls. Enzyme\u2010linked immunosorbent assay and real\u2010time fluorescence reverse transcription\u2010polymerase chain reaction were used for the measurement of protein and mRNA level, respectively. In addition, clinical periodontal parameters were also checked.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> AGEs strongly induced MMP\u20101 mRNA and protein expression in HGFs and in a time\u2010 and concentration\u2010dependent manner (<jats:italic>P<\/jats:italic> &lt;0.05). In gingival connective tissue, the level of both RAGE mRNA and NF\u2010\u03baB mRNA were higher in patients with periodontitis than in healthy controls (<jats:italic>P<\/jats:italic> &lt;0.05). There was significant correlation between the level of RAGE mRNA and NF\u2010\u03baB mRNA (<jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup> = 0.90, <jats:italic>P<\/jats:italic> &lt;0.05).<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> Accumulation of AGEs may upregulate the expression of MMP\u20101 by HGFs, which may play a role in the development of diabetes\u2010associated periodontitis, and RAGE\/NF\u2010\u03baB pathway may be involved in metabolism of MMP\u20101 in HGFs.<\/jats:p>","DOI":"10.1902\/jop.2011.100754","type":"journal-article","created":{"date-parts":[[2011,5,12]],"date-time":"2011-05-12T17:45:49Z","timestamp":1305222349000},"page":"119-126","source":"Crossref","is-referenced-by-count":45,"title":["Matrix Metalloproteinase\u20101 of Gingival Fibroblasts Influenced by Advanced Glycation End Products (AGEs) and Their Association With Receptor for AGEs and Nuclear Factor\u2010\u03baB in Gingival Connective 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