{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T06:20:28Z","timestamp":1791181228547,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2011,11,1]],"date-time":"2011-11-01T00:00:00Z","timestamp":1320105600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2010\/16605\u20100"],"award-info":[{"award-number":["2010\/16605\u20100"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:p>\n                    <jats:bold>Background:<\/jats:bold>\n                    We previously reported a higher expression of protease\u2010activated receptor\u20102 (PAR\n                    <jats:sub>2<\/jats:sub>\n                    ) together with higher interleukin (IL)\u20101\u03b1, IL\u20106, IL\u20108, and tumor necrosis factor\u2010\u03b1 levels, total proteolytic activity,\n                    <jats:italic>Porphyromonas gingivalis<\/jats:italic>\n                    (\n                    <jats:italic>Pg<\/jats:italic>\n                    ) prevalence, and neutrophil\u2010protease 3 messenger RNA (mRNA) expression in patients with chronic periodontitis compared to healthy control patients. The aim of the present study is to expand this observation by considering the site level according to the presence of\n                    <jats:italic>Pg<\/jats:italic>\n                    .\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods:<\/jats:bold>\n                    Microbiologic and gingival crevicular fluid samples were collected from patients with chronic periodontitis.\n                    <jats:italic>Pg<\/jats:italic>\n                    presence was evaluated by polymerase chain reaction and PAR\n                    <jats:sub>2<\/jats:sub>\n                    mRNA expression was evaluated by reverse\u2010transcription polymerase chain reaction. Total proteolytic activity in the crevicular fluid was analyzed by using a specific substrate benzoylarginine nitroanilide, and the proinflammatory mediators IL\u20101\u03b1, IL\u20106, IL\u20108, and tumor necrosis factor\u2010\u03b1 were analyzed by enzyme\u2010linked immunosorbent assay.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results:<\/jats:bold>\n                    In\n                    <jats:italic>Pg<\/jats:italic>\n                    \u2013positive periodontal sites, the mean probing depth and clinical attachment level, the prevalence of bleeding on probing sites, and crevicular fluid volume were higher (\n                    <jats:italic>P<\/jats:italic>\n                    &lt;0.05) compared to\n                    <jats:italic>Pg<\/jats:italic>\n                    \u2013negative sites. In addition, with the exception of IL\u20108, all other inflammatory mediators were positively (\n                    <jats:italic>P<\/jats:italic>\n                    &lt;0.05) associated with\n                    <jats:italic>Pg<\/jats:italic>\n                    presence.\n                    <jats:italic>Pg<\/jats:italic>\n                    presence was also positively associated with a higher proteolytic activity (\n                    <jats:italic>P<\/jats:italic>\n                    = 0.0037) and higher PAR\n                    <jats:sub>2<\/jats:sub>\n                    mRNA expression (\n                    <jats:italic>P<\/jats:italic>\n                    = 0.0271).\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusions:<\/jats:bold>\n                    We conclude that in chronic periodontitis, periodontal pockets presenting\n                    <jats:italic>Pg<\/jats:italic>\n                    show an upregulation of PAR\n                    <jats:sub>2<\/jats:sub>\n                    gene expression, and higher proinflammatory profile associated with advanced clinical destruction, therefore suggesting that\n                    <jats:italic>Pg<\/jats:italic>\n                    plays a pivotal role on PAR\n                    <jats:sub>2<\/jats:sub>\n                    \u2010mediated periodontal inflammation in humans.\n                  <\/jats:p>","DOI":"10.1902\/jop.2011.110073","type":"journal-article","created":{"date-parts":[[2011,4,5]],"date-time":"2011-04-05T20:59:26Z","timestamp":1302037166000},"page":"1596-1601","source":"Crossref","is-referenced-by-count":21,"title":["<i>Porphyromonas Gingivalis<\/i>\n                    is Associated With Protease\u2010Activated Receptor\u20102 Upregulation in Chronic Periodontitis"],"prefix":"10.1002","volume":"82","author":[{"given":"Jos\u00e9 Am\u00e9rico G.","family":"Fagundes","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Let\u00edcia D.","family":"Monoo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa Tubero","family":"Euz\u00e9bio Alves","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cl\u00e1udio Mendes","family":"Pannuti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheila Cavalca","family":"Cortelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Roberto","family":"Cortelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marinella","family":"Holzhausen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.18388\/abp.1996_4477"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0757.2000.2240108.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.2002.7.1.72"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910508400209"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.8.5121-5130.2001"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00028.2003"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/89945"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762005000900030"},{"key":"e_1_2_7_10_1","first-page":"3238","volume":"155","author":"Giacaman RA","year":"2009","journal-title":"Cleavage of protease\u2010activated receptors on an immortalized oral epithelial cell line by Porphyromonas gingivalis gingipains"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2006.050658"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034510373765"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1042\/CBI20090290"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-49-7-621"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1159\/000113041"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.09-146167"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00286.2003"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2009.07.004"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3141009"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2001.119025"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2006.04.015"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10067-006-0495-8"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2006.01317.x"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1203\/01.PDR.0000161416.63314.70"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2003.015156"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1136\/thorax.57.2.146"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1097\/00054725-200307000-00002"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI16913"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.22312"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2008.150722"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI29255"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.9.5315"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2007.04.002"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20052148"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2009.05.004"},{"key":"e_1_2_7_36_1","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1016\/S0031-6997(24)01493-5","article-title":"Proteinase\u2010activated receptors","volume":"53","author":"Macfarlane SR","year":"2001","journal-title":"Pharmacol Rev"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.4.1660-1665.1998"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.47679-0"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.2009.00507.x"}],"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2011.110073","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T13:07:38Z","timestamp":1760879258000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2011.110073"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,11]]},"references-count":38,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2011,11]]}},"alternative-id":["10.1902\/jop.2011.110073"],"URL":"https:\/\/doi.org\/10.1902\/jop.2011.110073","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,11]]}}}