{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T09:28:21Z","timestamp":1768555701943,"version":"3.49.0"},"reference-count":35,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2011,12,1]],"date-time":"2011-12-01T00:00:00Z","timestamp":1322697600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["20791352"],"award-info":[{"award-number":["20791352"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2011,12]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Clinically, severe cemental resorption is a rare consequence of periodontitis, although alveolar bone resorption by osteoclasts is one of the main pathologic changes. F\u2010spondin is a secreted neuronal glycoprotein that localizes to the cementum. F\u2010spondin is among the cementum\u2010specific factors in periodontal tissue that have been reported. However, the effects of F\u2010spondin on osteoclastogenesis have not yet been established. We examined the effects of F\u2010spondin on stages of osteoclastogenesis, migration, and differentiation in a mouse osteoclastic precursor model, RAW 264 cells.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> RAW 264 cells were treated with recombinant F\u2010spondin. Macrophage colony stimulating factor (M\u2010CSF)\u2013induced cell migration was examined by migration assay performed with cell culture inserts. Osteoclastic differentiation was measured by counting tartrate\u2010resistant acid phosphatase (TRAP)\u2013positive multinucleated cells.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> In a transmigration assay, F\u2010spondin significantly downregulated M\u2010CSF\u2013induced cell migration. Further, F\u2010spondin significantly reduced the number of receptor activator of nuclear factor\u2010kappa B ligand\u2013induced TRAP\u2010positive multinucleated cells. The receptor\u2010associated protein, an antagonist of the low\u2010density lipoprotein (LDL) receptor family, blocked the effects of F\u2010spondin on M\u2010CSF\u2013induced migration. The suppressive effect of F\u2010spondin on M\u2010CSF\u2013induced cell migration was blocked by knockdown of LDL receptor\u2013related protein 8 (LRP8), a member of the LDL receptor family.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> Our findings suggest that F\u2010spondin downregulates recruitment to the root side of periodontal tissue via LRP8 and inhibits differentiation of osteoclastic precursors. It is suggested that F\u2010spondin is essential to protect the root surface from resorption.<\/jats:p>","DOI":"10.1902\/jop.2011.110111","type":"journal-article","created":{"date-parts":[[2011,4,13]],"date-time":"2011-04-13T16:47:39Z","timestamp":1302713259000},"page":"1776-1783","source":"Crossref","is-referenced-by-count":12,"title":["F\u2010Spondin Inhibits Migration and Differentiation of Osteoclastic 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