{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T08:05:41Z","timestamp":1790841941640,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2012,7,1]],"date-time":"2012-07-01T00:00:00Z","timestamp":1341100800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2009\/02976\u20100"],"award-info":[{"award-number":["2009\/02976\u20100"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2009\/02846\u20109"],"award-info":[{"award-number":["2009\/02846\u20109"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2012,7]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> The purpose of this study is to investigate whether local inflammatory events, such as periodontal disease, are able to increase tumor necrosis factor\u2010alpha (TNF\u2010\u03b1) plasmatic concentration and decrease insulin sensitivity and insulin signaling in non\u2010diabetic rats.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Forty\u2010eight male Wistar rats (2 months old) were divided into two groups, with either ligature\u2010induced periodontal disease (LPD) or control conditions (CN). Experiments were performed in both groups 28 days after ligature placement. Plasmatic concentration of glycemia and TNF\u2010\u03b1 (n = 10) were analyzed by the glucose oxidase and enzyme\u2010linked immunosorbent assay method, respectively. Insulin sensitivity (n = 7) was measured using the insulin tolerance test. Insulin signal transduction (n = 7) was measured by pp185 tyrosine phosphorylation status in insulin\u2010sensitive tissues using the Western blotting method.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> The LPD group showed decreased insulin sensitivity (<jats:italic>P<\/jats:italic> &lt;0.05), although no glycemic alterations were noted (<jats:italic>P<\/jats:italic> &gt;0.05). TNF\u2010\u03b1 plasmatic concentration was higher in LPD rats compared to CN rats. In addition, a decrease in the pp185 tyrosine phosphorylation status was observed after insulin stimulus in both white adipose and skeletal muscle tissues of the LPD group compared with the CN group.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> LPD is able to cause alterations to both insulin signaling and insulin sensitivity, probably because of the elevation of TNF\u2010\u03b1 plasmatic concentration. Thus, the present results emphasize the importance of the prevention of local inflammatory diseases, such as periodontitis, to prevent diabetes mellitus.<\/jats:p>","DOI":"10.1902\/jop.2011.110349","type":"journal-article","created":{"date-parts":[[2011,11,17]],"date-time":"2011-11-17T02:00:03Z","timestamp":1321495203000},"page":"864-870","source":"Crossref","is-referenced-by-count":41,"title":["Periodontal Disease Decreases Insulin Sensitivity and Insulin Signaling"],"prefix":"10.1002","volume":"83","author":[{"given":"Natalia H.","family":"Colombo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisy J.","family":"Shirakashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando Y.","family":"Chiba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Sara de Lima 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