{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T17:55:56Z","timestamp":1780595756764,"version":"3.54.1"},"reference-count":65,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2012,9,1]],"date-time":"2012-09-01T00:00:00Z","timestamp":1346457600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> The release profile of 25% doxycycline (DOX) gel loaded on a biodegradable collagen membrane (COL) after 24% EDTA root surface etching was evaluated.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Thirty systemically healthy patients, each with at least one pair of contralateral interproximal intrabony defects \u22654 mm deep, along with an interproximal probing depth \u22656 mm and clinical attachment loss \u22654 mm, were randomized into two groups. Group 1 consisted of sites treated with open\u2010flap debridement followed by placement of DOX gel\u2010loaded COL (DOX\u2013COL), whereas group 2 sites were treated with flap surgery followed by the placement of DOX\u2013COL after EDTA etching of the exposed root surfaces (DOX\u2013COL + EDTA). Samples of gingival crevicular fluid were obtained 1, 3, 7, 14, and 21 days after surgery. Separation was performed, and quantitative measurements of DOX were taken with a high\u2010performance liquid chromatography. Clinical evaluation and follow\u2010up for 6 months were performed.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> At 21 days, DOX\u2013COL + EDTA group showed 5.3 \u03bcg\/mL value. However, no DOX was detected in samples of the DOX\u2013COL group. DOX\u2013COL + EDTA\u2010treated group retained more DOX during the periods of 3, 7, 10, and 14 days than did the DOX\u2013COL group.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> EDTA root surface etching could enhance DOX availability in the gingival crevicular fluid after its release from the collagen membrane.<\/jats:p>","DOI":"10.1902\/jop.2011.110476","type":"journal-article","created":{"date-parts":[[2011,12,19]],"date-time":"2011-12-19T13:18:19Z","timestamp":1324300699000},"page":"1086-1094","source":"Crossref","is-referenced-by-count":14,"title":["A Novel Approach to the Use of Doxycycline\u2010Loaded Biodegradable Membrane and EDTA Root Surface Etching in Chronic Periodontitis: A Randomized Clinical Trial"],"prefix":"10.1002","volume":"83","author":[{"given":"Ahmed Y.","family":"Gamal","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Radi M.","family":"Kumper","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,9]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1981.tb02039.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1984.tb01344.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"crossref","unstructured":"SanderL FrandsenEV ArnbjergD WarrerK KarringT. 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