{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T00:38:39Z","timestamp":1779928719125,"version":"3.53.1"},"reference-count":47,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,3,1]],"date-time":"2013-03-01T00:00:00Z","timestamp":1362096000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2013,3]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Chinese hamster ovary (CHO) cell\u2010derived recombinant human bone morphogenetic protein\u20102 (rhBMP\u20102) has been introduced for spine, long bone, and craniofacial indications. <jats:italic>Escherichia coli<\/jats:italic>\u2010 (<jats:italic>E. coli<\/jats:italic>) derived rhBMP\u20102 displays comparable efficacy to CHO cell\u2010derived rhBMP\u20102 in vitro and in small\u2010animal models. The objective of this study is to evaluate the efficacy of <jats:italic>E. coli\u2010<\/jats:italic>derived rhBMP\u20102 compared to the benchmark CHO cell\u2010derived rhBMP\u20102 using an established large\u2010animal model.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Contralateral, critical\u2010size supraalveolar peri\u2010implant defects in six adult male Hound Labrador mongrel dogs received CHO cell\u2010 or <jats:italic>E. coli\u2010<\/jats:italic>derived rhBMP\u20102 (0.2 mg\/mL) in an absorbable collagen sponge (ACS) carrier. In each quadrant, three dental implants were placed. A titanium mesh device was used to support space provision. The animals received fluorescent bone markers for qualitative evaluations. Animals were euthanized at 8 weeks for histopathologic and histometric evaluation.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Clinical healing included significant swelling, but none of the animals experienced wound dehiscences. CHO cell\u2010 and <jats:italic>E. coli\u2010<\/jats:italic>derived rhBMP\u20102 supported comparable bone formation (new bone area, 35.8 \u00b1 3.6 versus 30.1 \u00b1 2.2 mm<jats:sup>2<\/jats:sup>; bone density, 31.8% \u00b1 1.6% versus 35.6% \u00b1 2.5%; and osseointegration, 32.9% \u00b1 7.4% versus 33.7% \u00b1 8.1%) without statistically significant differences between treatments. Newly formed immature delicate trabecular bone in fibrovascular marrow filled the space underneath the titanium mesh and extended coronally above the mesh. Seroma formation was frequently observed. There were no discernable qualitative histologic differences between treatments.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> CHO cell\u2010 and <jats:italic>E. coli\u2010<\/jats:italic>derived rhBMP\u20102 in an ACS carrier appear equally effective at inducing local bone formation in support of dental implant osseointegration.<\/jats:p>","DOI":"10.1902\/jop.2012.110369","type":"journal-article","created":{"date-parts":[[2012,5,21]],"date-time":"2012-05-21T16:10:03Z","timestamp":1337616603000},"page":"415-422","source":"Crossref","is-referenced-by-count":22,"title":["Comparative Study of Chinese Hamster Ovary Cell Versus <i>Escherichia coli\u2010<\/i>Derived Bone Morphogenetic Protein\u20102 Using the Critical\u2010Size Supraalveolar Peri\u2010Implant Defect Model"],"prefix":"10.1002","volume":"84","author":[{"given":"Jaebum","family":"Lee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eui Nam","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Yoon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sung\u2010Min","family":"Chung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hari","family":"Prasad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristiano","family":"Susin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulf M.E.","family":"Wikesj\u00f6","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,3]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.150.3698.893"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.24.9484"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3201241"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1990.tb07376.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.24.9843"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.6.2220"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)88709-4"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1994.2508"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.3109\/08977199609034567"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7799(01)01665-1"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.2106\/00004623-199274050-00005"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.2106\/00004623-199712000-00003"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003086-199804000-00026"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.1998.13.9.1483"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.1991.01870220049009"},{"key":"e_1_2_6_17_1","first-page":"604","article-title":"Bone formation and reosseointegration in peri\u2010implantitis defects following surgical implantation of rhBMP\u20102","volume":"12","author":"Hanisch O","year":"1997","journal-title":"Int J Oral Maxillofac Implants"},{"key":"e_1_2_6_18_1","first-page":"785","article-title":"Bone formation and osseointegration stimulated by rhBMP\u20102 following subantral augmentation procedures in nonhuman primates","volume":"12","author":"Hanisch O","year":"1997","journal-title":"Int J Oral Maxillofac Implants"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0501.1997.080503.x"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0905-7161.2003.clr140617.x"},{"key":"e_1_2_6_21_1","article-title":"Sinus augmentation using rhBMP\u20102\/ACS in a mini\u2010pig model: relative efficacy of autogenous fresh particulate iliac bone grafts [published ahead of print January 26, 2012]","author":"Lee J","year":"2012","journal-title":"Clin Oral Implants Res"},{"key":"e_1_2_6_22_1","unstructured":"Federal Drug Administration.http:\/\/www.accessdata.fda.gov\/cdrh_docs\/pdf\/p000054a.pdf. 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