{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T00:24:05Z","timestamp":1791591845827,"version":"4.3.4"},"reference-count":39,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2012,10,1]],"date-time":"2012-10-01T00:00:00Z","timestamp":1349049600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2012,10]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Effects of <jats:italic>Fusobacterium nucleatum (F. nucleatum)<\/jats:italic> biofilm on epithelial cell proliferation, apoptotic cell death, and basement membrane constituent collagen IV production were examined in an organotypic dento\u2010epithelial (OD\u2010E) model.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> The OD\u2010E model was constructed by seeding keratinocytes on fibroblast\u2010containing collagen gels and by placing tooth pieces on top. A 3\u2010day\u2010old biofilm either a laboratory strain (American Type Culture Collection [ATCC] 25586) or a clinical strain (Anaerobe Helsinki Negative [AHN] 9508) of <jats:italic>F. nucleatum<\/jats:italic> was placed on the top of the model. The coculture was incubated \u226424 hours. The expression and localization of Ki\u201067, caspase\u20103, and collagen IV were examined by immunohistochemistry.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Hematoxylin and eosin staining showed epithelial migration and lateral sprouting into the connective tissue matrix in <jats:italic>F. nucleatum<\/jats:italic> OD\u2010E cocultures. The proliferation pattern of the in vitro dento\u2010epithelial junction was changed. In controls without bacterial challenge, the Ki\u201067 expression was abundant in the cells attached to the tooth, whereas in <jats:italic>F. nucleatum<\/jats:italic> biofilm\u2010treated cultures, the Ki\u201067\u2010expressing cells were more often in the connective tissue\u2010facing side of the epithelium. An apoptotic marker caspase\u20103 was expressed in controls and in <jats:italic>F. nucleatum<\/jats:italic> laboratory strain ATCC cocultures throughout the epithelium, in contrast to cultures treated with <jats:italic>F. nucleatum<\/jats:italic> clinical strain AHN, in which caspase\u20103 was absent. Collagen IV stainings were negative in both controls and <jats:italic>F. nucleatum<\/jats:italic> cocultures.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> <jats:italic>F. nucleatum<\/jats:italic> biofilm coculture with OD\u2010E model causes lateral sprouting of the epithelium with an altered epithelial proliferation pattern, resembling the histologic changes seen in vivo in the early pathogenesis of periodontal disease.<\/jats:p>","DOI":"10.1902\/jop.2012.110535","type":"journal-article","created":{"date-parts":[[2012,1,16]],"date-time":"2012-01-16T12:59:35Z","timestamp":1326718775000},"page":"1329-1335","source":"Crossref","is-referenced-by-count":15,"title":["<i>Fusobacterium nucleatum<\/i> Biofilm Induces Epithelial Migration in an Organotypic Model of Dento\u2010Gingival Junction"],"prefix":"10.1002","volume":"83","author":[{"given":"Marja 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