{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T12:26:18Z","timestamp":1771676778023,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100008376","name":"Helsingin ja Uudenmaan Sairaanhoitopiiri","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008376","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> The aims of the present study include: 1) to localize human neutrophil defensin\u20101 (HNP\u20101) through HNP\u20103 in gingiva and in neutrophil extract\u2010treated epithelial cell monolayers; 2) to determine the effects of HNP\u20101 on the epithelial cell viability, attachment, and spreading; and 3) to analyze the effect of HNP\u20101 on the bacterial adherence to epithelial cells.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> For localization of HNP\u20101 through HNP\u20103 in gingival tissue and in preincubated cell monolayers, immunohistochemical and immunocytochemical methods were used. Viability and proliferation of epithelial cells were determined with commercial kits after incubating the keratinocytes with different HNP\u20101 concentrations (low, 1 to 5 \u03bcg\/mL; moderate, 10 \u03bcg\/mL; high, 20 to 50 \u03bcg\/mL). Attachment and spreading of keratinocytes on fibronectin\u2010coated surfaces in the presence of HNP\u20101 were evaluated under microscope. Attachment of <jats:italic>Fusobacterium nucleatum<\/jats:italic> ATCC25586 and <jats:italic>Prevotella intermedia<\/jats:italic> ATCC25611 on keratinocytes preincubated with HNP\u20101 were determined with a standard antibiotic test.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> HNP\u20101 through HNP\u20103 localized in the junctional epithelium of clinically healthy gingiva and in the pocket epithelium of gingiva with periodontitis. When keratinocyte monolayers were incubated with neutrophil extracts, HNP\u20101 through HNP\u20103 were bound to the periphery of the growing cell colonies. In low HNP\u20101 concentrations, the keratinocyte proliferation enhanced. Moderate and high concentrations of HNP\u20101 increased the cellular death significantly. HNP\u20101 increased the attachment and spreading of keratinocytes on fibronectin\u2010coated surfaces and bacterial attachment to keratinocytes in a concentration\u2010dependent manner.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> HNP\u20101 plays a role in the integrity of keratinocytes by stimulating their proliferation, attachment, and spreading, whereas higher doses increase the bacterial attachment and keratinocyte death.<\/jats:p>","DOI":"10.1902\/jop.2012.120017","type":"journal-article","created":{"date-parts":[[2012,3,24]],"date-time":"2012-03-24T03:52:10Z","timestamp":1332561130000},"page":"126-133","source":"Crossref","is-referenced-by-count":42,"title":["Human Neutrophil Defensins and Their Effect on Epithelial Cells"],"prefix":"10.1002","volume":"84","author":[{"given":"Ulvi 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