{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T16:10:21Z","timestamp":1773850221220,"version":"3.50.1"},"reference-count":48,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2013,5,1]],"date-time":"2013-05-01T00:00:00Z","timestamp":1367366400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["K23DE019872"],"award-info":[{"award-number":["K23DE019872"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000072","name":"National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["F32DE021934"],"award-info":[{"award-number":["F32DE021934"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Periostin is a matricellular protein essential for tissue integrity and maturation and is believed to have a key function as a modulator of periodontal ligament (PDL) homeostasis. The aim of this study is to evaluate whether periodontal disease\u2010associated pathogen\u2010related virulence factors (endotoxins\/lipopolysaccharides [LPS]) and proinflammatory cytokines alter the expression of periostin in PDL cells.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Human PDL cultures were exposed to inflammatory mediators (tumor necrosis factor\u2010\u03b1 [TNF\u2010\u03b1]), bacterial virulence factors (<jats:italic>Porphyromonas gingivalis<\/jats:italic> LPS) or a combination in a biomechanically challenged environment. Culture conditions were applied for 24 hours, 4 days, and 7 days. Periostin and TGF\u2010\u03b2 inducible gene clone H3 (\u03b2IGH3) mRNA expression from cell lysates were analyzed. Periostin and \u03b2IGH3 proteins were also detected and semiquantified in both cell lysates and cell culture supernatants by Western blot. In addition, periostin localization by immunofluorescence was performed. Analysis of variance and Fisher tests were used to define the statistical differences among groups (<jats:italic>P<\/jats:italic> &lt;0.05).<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> In a mechanically challenged environment, periostin protein was more efficiently incorporated into the matrix compared to the non\u2010loaded controls (higher levels of periostin in the supernatant in the non\u2010loaded group). Interestingly, chronic exposure to proinflammatory cytokines and\/or microbial virulence factors significantly decreased periostin protein levels in the loaded cultures. There was greater variability on \u03b2IGH3 levels, and no particular pattern was clearly evident.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> Inflammatory mediators (TNF\u2010\u03b1) and bacterial virulence factors (<jats:italic>P. gingivalis<\/jats:italic> LPS) decrease periostin expression in human PDL fibroblasts. These results support a potential mechanism by which periostin alterations could act as a contributing factor during periodontal disease progression.<\/jats:p>","DOI":"10.1902\/jop.2012.120078","type":"journal-article","created":{"date-parts":[[2012,7,6]],"date-time":"2012-07-06T11:10:12Z","timestamp":1341573012000},"page":"694-703","source":"Crossref","is-referenced-by-count":36,"title":["Tumor Necrosis Factor\u2010\u03b1 and <i>Porphyromonas gingivalis<\/i> Lipopolysaccharides Decrease Periostin in Human Periodontal Ligament Fibroblasts"],"prefix":"10.1002","volume":"84","author":[{"given":"Miguel","family":"Padial\u2010Molina","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah L.","family":"Volk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan C.","family":"Rodriguez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julie T.","family":"Marchesan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pablo","family":"Galindo\u2010Moreno","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hector F.","family":"Rios","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,5]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2940271"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2010.07.001"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.25.24.11131-11144.2005"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.10453"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2008.070624"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.1999.14.7.1239"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2005.04.002"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910208101205"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12079-008-0023-5"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2148-10-30"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.1997.tb00189.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034510378423"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2010.03.013"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.155.2.831-838.1983"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.28.7.1551-1559.1990"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)67728-8"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1998.tb02419.x"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345000790120701"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0765.2002.90770.x"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S8756-3282(02)00673-7"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.modgep.2005.03.005"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.21224"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00429-005-0010-z"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-002-0664-2"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.10272"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2006.02.016"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.051961"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.088864"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.00098"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.a.20080"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2011.09.057"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.140202"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0765.2003.00602.x"},{"key":"e_1_2_6_35_1","unstructured":"RiosHF. 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