{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T06:55:10Z","timestamp":1771484110879,"version":"3.50.1"},"reference-count":41,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2013,10,1]],"date-time":"2013-10-01T00:00:00Z","timestamp":1380585600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Expression patterns of human \u03b2\u2010defensin\u20102 (HBD\u20102) mRNA or HBD\u20102 protein concentration and periodontal diseases have been a focus of scientific research. This study compares the salivary levels of HBD\u20102 protein concentration of healthy patients and patients with gingivitis and chronic periodontitis (CP) and correlates these levels with the presence of periodontopathogens.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> A total of 89 patients were enrolled in this study: 31 periodontally healthy, 27 with gingivitis, and 31 with CP. Plaque and gingival indices, probing depth, and clinical attachment level were measured. The presence of <jats:italic>Campylobacter rectus<\/jats:italic>, <jats:italic>Aggregatibacter actinomycetemcomitans<\/jats:italic>, <jats:italic>Porphyromonas gingivalis<\/jats:italic>, <jats:italic>Tannerella forsythia<\/jats:italic>, <jats:italic>Treponema denticola<\/jats:italic>, and <jats:italic>Prevotella intermedia<\/jats:italic> was evaluated qualitatively by conventional polymerase chain reaction. HBD\u20102 quantification in saliva was performed using an immune enzymatic assay. Frequency of periodontopathogens and HBD\u20102 protein concentration was assessed. Association between HBD\u20102 protein concentration (\u2265100 pg\/mL) and the simultaneous presence of one to two, three to four, or five to six periodontopathogens was tested.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Although periodontally healthy individuals and patients with gingivitis showed similar HBD\u20102 levels, the CP group displayed an increased level of HBD\u20102. <jats:italic>P. gingivalis<\/jats:italic>, <jats:italic>P. intermedia<\/jats:italic>, <jats:italic>T. forsythia<\/jats:italic>, and <jats:italic>T. denticola<\/jats:italic> were more prevalent in CP; however, their mere presence was not related to the increased levels of HBD\u20102 (Pearson correlation and multinomial logistic regression model).<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> Salivary HBD\u20102 protein concentration was higher in patients with CP compared with healthy individuals or patients with gingivitis. These different protein concentrations were not related to the frequency of periodontopathogens. Clinical inflammatory profile had a higher impact on salivary HBD\u20102 levels than bacteria.<\/jats:p>","DOI":"10.1902\/jop.2012.120321","type":"journal-article","created":{"date-parts":[[2012,11,23]],"date-time":"2012-11-23T08:23:25Z","timestamp":1353659005000},"page":"1445-1453","source":"Crossref","is-referenced-by-count":34,"title":["Influence of Periodontal Status and Periodontopathogens on Levels of Oral Human \u03b2\u2010Defensin\u20102 in Saliva"],"prefix":"10.1002","volume":"84","author":[{"given":"Alexandre L.","family":"Pereira","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gilson C.","family":"Franco","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheila C.","family":"Cortelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Davi 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