{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T07:27:29Z","timestamp":1790321249389,"version":"4.1.0"},"reference-count":27,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,7,1]],"date-time":"2013-07-01T00:00:00Z","timestamp":1372636800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Most available periodontal studies regarding the endothelial cell (EC) were investigated by using human umbilical vein endothelial cells (HUVECs); however, ECs can display remarkable heterogeneity in vascular beds of different origins. The aim of the present study, therefore, is to characterize microvascular ECs isolated from periodontal tissue and investigate their growth and gene expression compared to HUVECs (macrovascular).<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Periodontal ligament ECs (PDL\u2010ECs) and gingiva ECs (G\u2010ECs) were isolated by coupling to monoclonal anti\u2010CD31 antibody magnetic beads. Both PDL\u2010ECs and G\u2010ECs were characterized to definitively demonstrate that the culture represented pure ECs. Their growth was determined by resazurin reduction assay. Interleukin (IL)\u20108, intercellular adhesion molecule 1 (ICAM\u20101), and E\u2010selectin gene expression were determined by real\u2010time quantitative reverse\u2010transcription polymerase chain reaction after treatment with <jats:italic>Escherichia coli<\/jats:italic> lipopolysaccharide (LPS).<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> PDL\u2010ECs and G\u2010ECs revealed specific EC characteristics but formed tube\u2010like structures and had slower growth rates than HUVECs. After <jats:italic>E. coli<\/jats:italic> LPS treatment, PDL\u2010ECs and G\u2010ECs showed similar dose\u2010dependently increased levels of IL\u20108, ICAM\u20101, and E\u2010selectin mRNA expression; however, their expressions were in contrast to HUVECs. PDL\u2010ECs and G\u2010ECs showed obviously increased ICAM\u20101 mRNA expression, whereas HUVECs showed markedly increased E\u2010selectin mRNA expression after treatment with 0.1 \u03bcg\/mL <jats:italic>E. coli<\/jats:italic> LPS.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> ECs isolated from periodontal tissue show different growth and gene expression from those of HUVECs. Thus, these microvascular ECs appear to be a more valuable in vitro model system than HUVECs (macrovascular) to further study pathogenesis and angiogenesis of periodontal disease.<\/jats:p>","DOI":"10.1902\/jop.2012.120453","type":"journal-article","created":{"date-parts":[[2012,9,24]],"date-time":"2012-09-24T22:47:20Z","timestamp":1348526840000},"page":"1002-1009","source":"Crossref","is-referenced-by-count":9,"title":["Comparative Studies on Microvascular Endothelial Cells Isolated From Periodontal 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