{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:30:35Z","timestamp":1787023835198,"version":"build-2736575974"},"reference-count":24,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2013,11,1]],"date-time":"2013-11-01T00:00:00Z","timestamp":1383264000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:p>\n                    <jats:bold>Background:<\/jats:bold>\n                    Matrix metalloproteinase\u201013 (\n                    <jats:italic>MMP\u201013<\/jats:italic>\n                    ) is an important enzyme for the modulation of bone turnover and gingival recession. Elevated levels of\n                    <jats:italic>MMP\u201013<\/jats:italic>\n                    are associated with alveolar bone resorption, periodontal ligament breakdown, and gingival attachment loss, which are the clinical symptoms of periodontal disease. Evidence continues to suggest that periodontal disease contributes to oral tissue breakdown and is linked to numerous systemic conditions. Triclosan (TCN) is a long\u2010standing, proven antibacterial and anti\u2010inflammatory agent found in the only Food and Drug Administration\u2013approved dentifrice for the treatment of plaque and gingivitis.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods:<\/jats:bold>\n                    This study examines the inhibitory effects of TCN on lipopolysaccharide\u2010, parathyroid hormone (PTH)\u2010, and prostaglandin E\n                    <jats:sub>2<\/jats:sub>\n                    (PGE\n                    <jats:sub>2<\/jats:sub>\n                    )\u2010induced expression of\n                    <jats:italic>MMP\u201013<\/jats:italic>\n                    in UMR 106\u201001 cells, an osteoblastic osteosarcoma cell line. The cells were stimulated with PTH or PGE\n                    <jats:sub>2<\/jats:sub>\n                    to induce\n                    <jats:italic>MMP\u201013<\/jats:italic>\n                    mRNA expression, and real\u2010time reverse transcription\u2010polymerase chain reaction was performed to determine gene expression levels. Western blot analysis assessed the presence or absence of protein degradation or inhibition of protein synthesis.\n                    <jats:italic>MMP\u201013<\/jats:italic>\n                    promoter reporter assay was used to explore possible direct effects of TCN on the\n                    <jats:italic>MMP\u201013<\/jats:italic>\n                    promoter.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results:<\/jats:bold>\n                    TCN significantly reduced PTH or PGE\n                    <jats:sub>2<\/jats:sub>\n                    elevated expression of\n                    <jats:italic>MMP\u201013<\/jats:italic>\n                    in osteoblastic cells without affecting basal levels of the mRNA. Surprisingly, TCN enhanced the expression of\n                    <jats:italic>c\u2010fos<\/jats:italic>\n                    and\n                    <jats:italic>amphiregulin<\/jats:italic>\n                    mRNA. A promoter assay indicated that TCN directly inhibits the activation of the PTH\u2010responsive minimal promoter of\n                    <jats:italic>MMP\u201013<\/jats:italic>\n                    .\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion:<\/jats:bold>\n                    The present study appears to have identified a nuclear mechanism of action of TCN that accounts for the ability of TCN to inhibit PTH\u2010 or PGE\n                    <jats:sub>2<\/jats:sub>\n                    \u2010induced\n                    <jats:italic>MMP\u201013<\/jats:italic>\n                    expression in osteoblastic cells.\n                  <\/jats:p>","DOI":"10.1902\/jop.2013.120154","type":"journal-article","created":{"date-parts":[[2013,1,31]],"date-time":"2013-01-31T10:08:52Z","timestamp":1359626932000},"page":"1683-1689","source":"Crossref","is-referenced-by-count":6,"title":["Triclosan Blocks\n                    <i>MMP<\/i>\n                    <i>\u201013<\/i>\n                    Expression in Hormone\u2010Stimulated Osteoblasts"],"prefix":"10.1002","volume":"84","author":[{"given":"Virginia Monsul","family":"Barnes","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emi","family":"Shimizu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teruyo","family":"Nakatani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven","family":"Jefcoat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anatoliy","family":"Vasilov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Qin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola C.","family":"Partridge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,11]]},"reference":[{"issue":"7","key":"e_1_2_7_2_1","first-page":"4","article-title":"Inflammation, periodontal diseases, and systemic health","volume":"25","author":"Gaffar A","year":"2004","journal-title":"Compend Contin Educ Dent"},{"key":"e_1_2_7_3_1","unstructured":"XuT DeshmukhM Monsul\u2010BarnesV TrivediH CumminsD.Effectiveness of a triclosan\/copolymer dentifrice on microbiological and inflammatory parameters.Compend Cont Educ Dent2004;25(Suppl. 1):46\u201053."},{"key":"e_1_2_7_4_1","unstructured":"SocranskySS HaffajeeAD. 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