{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:41:02Z","timestamp":1788871262875,"version":"build-2803163510"},"reference-count":17,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,6,1]],"date-time":"2014-06-01T00:00:00Z","timestamp":1401580800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:p>\n                    <jats:bold>Background:<\/jats:bold>\n                    Monocyte subsets with low CD14 expression that coexpress CD16 (CD14+CD16+) are called non\u2010classic or hyperinflammatory monocytes. Previous studies have reported an increase in the percentage of CD14+CD16+ monocytes in the peripheral blood of patients with chronic periodontitis (CP). To our knowledge, there are no reports demonstrating the presence of CD14+CD16+ monocyte\u2013derived macrophages (MDMs) in the gingival tissue. The objective of this study is to identify the proportion of non\u2010classic (CD14+CD16+) monocytes\/macrophages in peripheral blood and gingiva of healthy individuals and patients with CP.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods:<\/jats:bold>\n                    A total of 60 individuals (n = 30 per group) were recruited for the study. Group 1 included 30 individuals with healthy gingiva, and group 2 included 30 patients with CP. Direct immunofluorescent staining was done in 200 \u03bcL whole\u2010blood and single\u2010cell suspensions obtained from gingival tissue, with fluorochrome\u2010conjugated monoclonal antibodies against CD14, CD16, and human leukocyte antigen\u2010DR (HLA\u2010DR), and subjected to flow cytometric analysis.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results:<\/jats:bold>\n                    The mean percentage of CD14+CD16+ monocytes in the peripheral blood of healthy individuals was 9.10% \u00b1 1.39%, and for patients with CP it was 14.18% \u00b1 2.69% (\n                    <jats:italic>P<\/jats:italic>\n                    &lt;0.05). The mean percentage of CD14+CD16+ MDMs in the gingival tissue of healthy individuals was found to be 0.93% \u00b1 0.33%, whereas in patients with CP, it was 1.92% \u00b1 0.78% (\n                    <jats:italic>P<\/jats:italic>\n                    &lt;0.01). Non\u2010classic monocytes\/macrophages showed a high median fluorescent intensity for HLA\u2010DR (DR++).\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion:<\/jats:bold>\n                    This study demonstrates an increased proportion of CD14+CD16+HLA\u2010DR++ monocytes\/macrophages in the peripheral blood and gingiva of patients with CP.\n                  <\/jats:p>","DOI":"10.1902\/jop.2013.120658","type":"journal-article","created":{"date-parts":[[2013,9,3]],"date-time":"2013-09-03T14:14:33Z","timestamp":1378217673000},"page":"852-858","source":"Crossref","is-referenced-by-count":34,"title":["Comparison of the Proportion of Non\u2010Classic (CD14+CD16+) Monocytes\/Macrophages in Peripheral Blood and Gingiva of Healthy Individuals and Patients With Chronic Periodontitis"],"prefix":"10.1002","volume":"85","author":[{"given":"Raghunathan","family":"Jagannathan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vamsi","family":"Lavu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suresh Ranga","family":"Rao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,6]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1998.3.1.40"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-02-258558"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.6.2782-2790.1998"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201141907"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2008.05.035"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2004.00713.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1999.4.1.1"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.7.3864"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.1997.tb00189.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s002239900473"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0320(19970415)30:2<72::AID-CYTO2>3.0.CO;2-F"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1989.tb00862.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1996.67.10s.1123"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.10545"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1160\/TH04-02-0095"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.22104"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.7.3536"}],"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2013.120658","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T10:41:29Z","timestamp":1760870489000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2013.120658"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,6]]},"references-count":17,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2014,6]]}},"alternative-id":["10.1902\/jop.2013.120658"],"URL":"https:\/\/doi.org\/10.1902\/jop.2013.120658","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,6]]}}}