{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:34:10Z","timestamp":1760920450244,"version":"build-2065373602"},"reference-count":50,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,3,1]],"date-time":"2014-03-01T00:00:00Z","timestamp":1393632000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:p><jats:bold>Background<\/jats:bold><jats:bold>:<\/jats:bold> Systemic hyperglycemia is clearly related to severity of periodontitis, meaning that periodontitis can be exacerbated by diabetes mellitus (DM). However, the biologic mechanisms responsible for this relationship remain unclear. Thus, in this study, the global gene expression in gingival tissue with periodontitis is profiled in Zucker diabetic fatty (ZDF) rats compared with Zucker normoglycemic littermates (Lean).<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> At age 8 weeks, ZDF and Lean rats received ligature placement around the maxillary right second molar. At 0, 14, 28, 42, and 56 days after ligature placement, the maxilla around the molar was analyzed using microcomputed tomography. Two and 7 days after ligature placement, total RNA in the gingival tissue was isolated, and gene expression analysis was conducted using a rat oligo array. To validate the microarray findings, the selected genes were analyzed using real\u2010time quantitative polymerase chain reaction assays.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> There was a significant difference regarding the average amount of bone resorption between ZDF and Lean rats from days 14 to 56. On day 2, it was found that 113 genes were regulated (20 upregulated, 93 downregulated) in the presence of DM. Lipopolysaccharide binding protein (LBP) messenger RNA (mRNA) expression was significantly higher in gingival tissue with periodontitis from ZDF rats compared with that from Lean rats. On day 7, interleukin (IL)\u201010 and IL\u201024 mRNA levels were significantly lower, whereas IL\u20102 level was significantly elevated.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> The results of this study indicate that the role of DM in modulating bone breakdown related to periodontitis may involve an increased level of LBP and reduced levels of T\u2010helper 2 cytokines.<\/jats:p>","DOI":"10.1902\/jop.2013.120705","type":"journal-article","created":{"date-parts":[[2013,6,3]],"date-time":"2013-06-03T11:54:23Z","timestamp":1370260463000},"page":"455-464","source":"Crossref","is-referenced-by-count":10,"title":["Altered Gene Expression in Gingival Tissues and Enhanced Bone Loss in Rats With Diabetes With Experimental Periodontitis"],"prefix":"10.1002","volume":"85","author":[{"given":"Kyoko","family":"Soboku","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeshi","family":"Kikuchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sachiko","family":"Fujita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroaki","family":"Takeda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keiko","family":"Naruse","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsuaki","family":"Matsubara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshihide","family":"Noguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,3]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.2001.6.1.99"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1988.59.1.23"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2011-3397"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-011-2342-y"},{"key":"e_1_2_6_6_1","first-page":"26","article-title":"Effect of periodontal therapy on metabolic control and an inflammatory mediator in type 2 diabetic subjects: A report on 17 consecutive cases","volume":"14","author":"Serrano C","year":"2012","journal-title":"J Int Acad Periodontol"},{"key":"e_1_2_6_7_1","doi-asserted-by":"crossref","first-page":"913","DOI":"10.4049\/jimmunol.143.3.913","article-title":"Involvement of class II MHC molecules in the LPS\u2010induction of IL\u20101\/TNF secretions by human monocytes. 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