{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T10:10:20Z","timestamp":1760868620616,"version":"build-2065373602"},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,2,1]],"date-time":"2014-02-01T00:00:00Z","timestamp":1391212800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> <jats:italic>Porphyromonas gingivalis<\/jats:italic> lipid A heterogeneity modulates cytokine expression in human cells. This study investigates the effects of two lipid A isoforms of <jats:italic>P. gingivalis<\/jats:italic>, lipopolysaccharide (LPS)<jats:sub>1435\/1449<\/jats:sub> and LPS<jats:sub>1690<\/jats:sub>, on the secretion of proinflammatory and regulatory cytokines in total blood cultures from patients with and without chronic periodontitis (CP).<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> A cross\u2010sectional study was conducted in 38 systemically healthy individuals divided in two groups: 1) the CP group (n = 19), in which patients were diagnosed with CP; and 2) the no periodontitis (NP) group (n = 19), which included control patients without CP. Blood samples were collected from all patients, and whole\u2010blood cell cultures (WBCCs) were stimulated for 48 hours with <jats:italic>P. gingivalis<\/jats:italic> LPS<jats:sub>1435\/1449<\/jats:sub> and LPS<jats:sub>1690<\/jats:sub> and <jats:italic>Escherichia coli<\/jats:italic> LPS. Unstimulated WBCCs served as negative controls. The secretion of interferon\u2010\u03b3 (IFN\u2010\u03b3), interleukin\u201010 (IL\u201010), and transforming growth factor\u2010\u03b2 (TGF\u2010\u03b2) was detected in WBCC supernatants by enzyme\u2010linked immunosorbent assays.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> <jats:italic>E. coli<\/jats:italic> LPS significantly increased the expression of all cytokines in WBCCs from both the NP and CP groups when compared to non\u2010stimulated cells (control treatment). <jats:italic>P. gingivalis<\/jats:italic> LPS preparations increased IFN\u2010\u03b3 levels in the CP group but not in the NP group when compared with controls (<jats:italic>P<\/jats:italic> &lt;0.05). <jats:italic>P. gingivalis<\/jats:italic> LPS preparations also increased IL\u201010 and TGF\u2010\u03b2 levels in both CP and NP groups, but <jats:italic>P. gingivalis<\/jats:italic> LPS<jats:sub>1690<\/jats:sub> showed a three\u2010fold increase on IL\u201010 production in the NP group (<jats:italic>P<\/jats:italic> &lt;0.05) when compared to <jats:italic>P. gingivalis<\/jats:italic> LPS<jats:sub>1435\/144<\/jats:sub>.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> These data demonstrate that WBCC cell populations obtained from healthy individuals and patients with CP may differ in the cytokine response to <jats:italic>P. gingivalis<\/jats:italic> but not <jats:italic>E. coli<\/jats:italic> LPS. This is consistent with the notion that CP alters the systemic WBCC response and that this can be detected by the different <jats:italic>P. gingivalis<\/jats:italic> LPS structures.<\/jats:p>","DOI":"10.1902\/jop.2013.120735","type":"journal-article","created":{"date-parts":[[2013,9,3]],"date-time":"2013-09-03T14:14:33Z","timestamp":1378217673000},"source":"Crossref","is-referenced-by-count":8,"title":["Whole\u2010Blood Cultures From Patients With Chronic Periodontitis Respond Differently to <i>Porphyromonas gingivalis<\/i> but not <i>Escherichia coli<\/i> Lipopolysaccharide"],"prefix":"10.1002","volume":"85","author":[{"given":"Getulio","family":"Nogueira\u2010Filho","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno Trevisan","family":"Rosa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia 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