{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T02:47:25Z","timestamp":1791859645718,"version":"4.3.4"},"reference-count":52,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2014,8,1]],"date-time":"2014-08-01T00:00:00Z","timestamp":1406851200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01\u2010DE013725S"],"award-info":[{"award-number":["R01\u2010DE013725S"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000097","name":"National Center for Research Resources","doi-asserted-by":"publisher","award":["UL1RR024986"],"award-info":[{"award-number":["UL1RR024986"]}],"id":[{"id":"10.13039\/100000097","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100002050","name":"Delta Dental Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100002050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Little is known about the release of apoptotic proteins during periodontal breakdown. This pilot study investigates the presence of factors associated with apoptosis in serum, saliva, and gingival crevicular fluid (GCF) and their association with periodontal disease severity and activity.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> GCF, whole saliva, and serum were obtained from 47 adult patients with chronic periodontitis (CP) and 10 healthy controls. Clinical measurements, including probing depth (PD), clinical attachment level (CAL), and radiographs, were used to classify patients into healthy, mild, and moderate\/severe CP groups. Enzyme\u2010linked immunosorbent assays were used to measure apoptosis or DNA fragmentation in GCF and active caspase\u20103, soluble Fas (sFas), and sFas ligand (sFasL) in saliva and serum. Western immunoblotting was used to detect Fas, FasL, sFasL, and caspase\u20103 expression in GCF.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> DNA fragmentation was positively correlated with PD and CAL regardless of patient disease status (<jats:italic>P<\/jats:italic> &lt;0.001). sFas and sFasL were present in saliva and serum, but there were no differences between groups. In GCF, the greater odds of detecting Fas, sFasL, and caspase\u20103 increased with increasing PD and CAL (<jats:italic>P<\/jats:italic> &lt;0.05). In addition, sites with inflammation and PD \u22655 mm had significantly greater odds of exhibiting Fas, sFasL, and caspase\u20103 expression compared with sites without inflammation and PD &lt;5 mm (<jats:italic>P<\/jats:italic> &lt;0.05). Caspase\u20103 was not detected in saliva or serum. At the patient level, only FasL and disease status were significantly correlated (<jats:italic>P<\/jats:italic> &lt;0.05).<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> Factors associated with apoptosis were detected in GCF in patients with CP.<\/jats:p>","DOI":"10.1902\/jop.2013.130095","type":"journal-article","created":{"date-parts":[[2013,12,23]],"date-time":"2013-12-23T02:31:22Z","timestamp":1387765882000},"page":"1086-1095","source":"Crossref","is-referenced-by-count":30,"title":["The Role of Factors Associated With Apoptosis in Assessing Periodontal Disease Status"],"prefix":"10.1002","volume":"85","author":[{"given":"Heba","family":"Abuhussein","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jill D.","family":"Bashutski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darya","family":"Dabiri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sindhu","family":"Halubai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary","family":"Layher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Klausner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huwaida","family":"Makhoul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvonne","family":"Kapila","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345990780060601"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9969(99)00020-5"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.103.2_Supplement.99S"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7878464"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.162.12.7140"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.270.5239.1189"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345960750060501"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051X.1999.260810.x"},{"key":"e_1_2_7_10_1","first-page":"1605","article-title":"Immunohistochemical analysis of in vivo patterns of expression of CPP32 (Caspase\u20103), a cell death protease","volume":"57","author":"Krajewska M","year":"1997","journal-title":"Cancer Res"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v10.i21.3151"},{"key":"e_1_2_7_12_1","first-page":"3053","article-title":"Relationship of oncogenes (sFas, Bcl\u20102) and cytokines (IL\u201010, alfa\u2010TNF) with the activity of systemic lupus erythematosus","volume":"21","author":"Miret C","year":"2001","journal-title":"Anticancer Res"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/00019501-199906000-00003"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004872-200205000-00024"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-005-1018-9"},{"key":"e_1_2_7_16_1","first-page":"1282","article-title":"Increased soluble CD95 (sFas\/CD95) serum level correlates with poor prognosis in melanoma patients","volume":"7","author":"Ugurel S","year":"2001","journal-title":"Clin Cancer Res"},{"key":"e_1_2_7_17_1","first-page":"311","article-title":"Plasma soluble Fas ligand concentration: Decrease in elderly men and increase in patients with gastric carcinoma","volume":"8","author":"Ichikura T","year":"2001","journal-title":"Oncol Rep"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10067-004-0909-4"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1053\/jhep.2001.28229"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/00002281-199905000-00007"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.11.5190-5195.1998"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2005.09.020"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2009.080480"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1983.tb01278.x"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1212\/01.wnl.0000249150.98891.d1"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2002.73.10.1101"},{"key":"e_1_2_7_27_1","first-page":"7","article-title":"Periodontal risk assessment (PRA) for patients in supportive periodontal therapy (SPT)","volume":"1","author":"Lang NP","year":"2003","journal-title":"Oral Health Prev Dent"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1990.tb01070.x"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1990.tb01071.x"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034511399908"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-9-280"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.53.1.155"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2009.08.014"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051X.2003.00350.x"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.25.1.427-439.1979"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2041-1014.2010.00576.x"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.06371-11"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910608500606"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1992.63.9.748"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2041-1014.2011.00609.x"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051X.2002.291203.x"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2005.00803.x"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1006\/cimm.1998.1432"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2008.01197.x"},{"key":"e_1_2_7_45_1","first-page":"915","article-title":"Detection of the soluble form of Fas ligand (sFasL) and sFas in the saliva from patients with Sj\u00f6gren's syndrome","volume":"23","author":"Nakamura H","year":"2005","journal-title":"Clin Exp Rheumatol"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2005.76.5.737"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.20411"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-11-4"},{"key":"e_1_2_7_49_1","first-page":"451","article-title":"Inhibition of human rheumatoid arthritis synovial cell survival by hecogenin and tigogenin is associated with increased apoptosis, p38 mitogen\u2010activated protein kinase activity and upregulation of cyclooxygenase\u20102","volume":"20","author":"Liagre B","year":"2007","journal-title":"Int J Mol Med"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2010.08.628"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.2334\/josnusd.44.35"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cden.2005.03.009"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034510380414"}],"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2013.130095","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T11:56:09Z","timestamp":1760874969000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2013.130095"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,8]]},"references-count":52,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2014,8]]}},"alternative-id":["10.1902\/jop.2013.130095"],"URL":"https:\/\/doi.org\/10.1902\/jop.2013.130095","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,8]]}}}