{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T20:18:19Z","timestamp":1778876299171,"version":"3.51.4"},"reference-count":42,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,2,1]],"date-time":"2014-02-01T00:00:00Z","timestamp":1391212800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["181271150"],"award-info":[{"award-number":["181271150"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> The mechanism by which <jats:italic>Porphyromonas gingivalis<\/jats:italic> regulates intracellular adhesion molecule 1 (ICAM\u20101) expression in human periodontal ligament cells (hPDLCs) and human gingival fibroblasts (hGFs) is unknown. The aim of this study is to investigate whether nucleotide binding oligomerization domain\u2010containing protein (NOD) 1 and NOD2 are involved in this process and the clinical significance of ICAM\u20101 in periodontitis.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> hPDLCs and hGFs were treated with <jats:italic>P. gingivalis<\/jats:italic>, <jats:sc>l<\/jats:sc>\u2010Ala\u2010\u03b3\u2010<jats:sc>d<\/jats:sc>\u2010glutamyl\u2010mesodiaminopimelic acid (an agonist for NOD1), and muramyl dipeptide (an agonist for NOD2). Alternatively, cells transfected with small interfering RNA targeting NOD1and NOD2 were treated with <jats:italic>P. gingivalis<\/jats:italic>. ICAM\u20101, NOD1, and NOD2 were detected at mRNA and protein levels. In addition, clinical examinations were performed in 30 healthy controls and 40 patients with chronic periodontitis (CP) before and after treatment, and serum\u2010soluble ICAM\u20101 (sICAM\u20101) levels in these individuals were detected by enzyme\u2010linked immunosorbent assay.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> This study shows that <jats:italic>P. gingivalis<\/jats:italic> caused an increase in ICAM\u20101, NOD1, and NOD2 expression in periodontal fibroblasts. There was a linear correlation between ICAM\u20101 and NOD1 and NOD2 levels. Activation of NOD1 and NOD2 by the specific agonist led to the upregulation of ICAM\u20101, whereas knocking down NOD1 and NOD2 caused a reduction in <jats:italic>P. gingivalis<\/jats:italic>\u2013induced ICAM\u20101 production. Furthermore, sICAM\u20101 levels were higher in patients with CP than in healthy controls and were positively related to the clinical periodontal parameters. After periodontal treatment, sICAM\u20101 levels decreased significantly.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> The present results indicate that sICAM\u20101 levels are correlated to the severity of periodontitis. NOD1 and NOD2 mediate <jats:italic>P. gingivalis<\/jats:italic>\u2013induced ICAM\u20101 production in periodontal fibroblasts. NOD1 and NOD2 could be considered potential targets for periodontal therapy.<\/jats:p>","DOI":"10.1902\/jop.2013.130152","type":"journal-article","created":{"date-parts":[[2013,5,20]],"date-time":"2013-05-20T11:59:19Z","timestamp":1369051159000},"page":"358-368","source":"Crossref","is-referenced-by-count":30,"title":["Intracellular Adhesion Molecule\u20101 Is Regulated by <i>Porphyromonas gingivalis<\/i> Through Nucleotide Binding Oligomerization Domain\u2010Containing Proteins 1 and 2 Molecules in Periodontal 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