{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T08:05:13Z","timestamp":1768464313717,"version":"3.49.0"},"reference-count":36,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2014,7,1]],"date-time":"2014-07-01T00:00:00Z","timestamp":1404172800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007197","name":"U.S. Public Health Service","doi-asserted-by":"publisher","award":["DE15566"],"award-info":[{"award-number":["DE15566"]}],"id":[{"id":"10.13039\/100007197","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007197","name":"U.S. Public Health Service","doi-asserted-by":"publisher","award":["DE19938"],"award-info":[{"award-number":["DE19938"]}],"id":[{"id":"10.13039\/100007197","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007197","name":"U.S. Public Health Service","doi-asserted-by":"publisher","award":["RR00533 (TEVD)"],"award-info":[{"award-number":["RR00533 (TEVD)"]}],"id":[{"id":"10.13039\/100007197","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Diacylglycerol (DAG), levels of which are tightly regulated by diacylglycerol kinases (DGKs), is a lipid mediator linked to key biologic functions. Members of the DGK family undergo alternative splicing, generating the protein diversity necessary to control different intracellular DAG pools. DGK\u03b1 function is altered in polymorphonuclear neutrophils (PMNs) of patients with localized aggressive periodontitis (LAgP), suggesting a genetic basis. Here, the authors assess DGK\u03b1 spliced transcripts in human LAgP neutrophils.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> In an expression library of a patient with LAgP, PMNs were screened for different DGK\u03b1 transcripts. Real\u2010time polymerase chain reaction and in vitro expression assays were performed to assess the fate of different transcripts on protein translocation and superoxide production in human leukemia cells (HL\u201060) and COS\u20107 cells.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> A DGK\u03b1 transcript that lacks exon 10 (DGK\u03b1\u039410) and generates a premature stop codon and a truncated protein was identified as being upregulated in LAgP neutrophils. In vitro assays revealed that DGK\u03b1\u039410 translocation occurred even in the absence of important regulatory motifs. Transfection of HL\u201060 neutrophil\u2010like cells with the DGK\u03b1\u039410 spliced variant induced an increase in the stimulated production of superoxide anion replicating the phenotype of LAgP PMNs.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> DGK\u03b1\u039410 can act as a dominant\u2010negative transcript that can modulate superoxide production and provides an example of genetic regulation of the inflammatory response that may be relevant to human inflammatory diseases such as LAgP.<\/jats:p>","DOI":"10.1902\/jop.2013.130468","type":"journal-article","created":{"date-parts":[[2013,10,30]],"date-time":"2013-10-30T14:52:04Z","timestamp":1383144724000},"page":"934-943","source":"Crossref","is-referenced-by-count":11,"title":["Alternative Splicing Generates a Diacylglycerol Kinase \u03b1 Transcript That Acts as a Dominant\u2010Negative Modulator of Superoxide Production in Localized Aggressive Periodontitis"],"prefix":"10.1002","volume":"85","author":[{"suffix":"Jr.","given":"Eraldo L.","family":"Batista","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alpdogan I.","family":"Kantarci","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hatice","family":"Hasturk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas E.","family":"Van 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