{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:44:46Z","timestamp":1760921086139,"version":"build-2065373602"},"reference-count":35,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2014,12,1]],"date-time":"2014-12-01T00:00:00Z","timestamp":1417392000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2010\/16605\u20100"],"award-info":[{"award-number":["2010\/16605\u20100"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Protease activated receptor\u20101 (PAR<jats:sub>1<\/jats:sub>) activation by thrombin may play a role in repair and homeostasis of periodontal tissues. The main objective of this study is to investigate PAR<jats:sub>1<\/jats:sub> expression in patients with periodontitis, before and after non\u2010surgical periodontal treatment, and to associate its expression with the presence of inflammatory biomarkers and PAR<jats:sub>2<\/jats:sub> expression.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Gingival crevicular fluid (GCF) samples and clinical parameters, including probing depth, clinical attachment level, bleeding on probing, and gingival and plaque indices, were collected from periodontally healthy individuals and patients with moderate chronic periodontitis (CP) before and 6 weeks after periodontal non\u2010surgical treatment. PAR<jats:sub>1<\/jats:sub> and PAR<jats:sub>2<\/jats:sub> messenger RNA (mRNA) at the GCF were evaluated by quantitative polymerase chain reaction (qPCR). Flow cytometry analysis identified the GCF PAR<jats:sub>1<\/jats:sub>\u2010expressing cells. GCF inflammatory biomarkers were also determined.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Clinical parameters were significantly improved after therapy (<jats:italic>P<\/jats:italic> &lt;0.01). The qPCR analysis showed that, before therapy, PAR<jats:sub>1<\/jats:sub> mRNA levels in CP were similar to controls. Periodontal treatment led to increased PAR<jats:sub>1<\/jats:sub> expression in CP (<jats:italic>P<\/jats:italic> &lt;0.05). PAR<jats:sub>1<\/jats:sub> expression was inversely correlated to PAR<jats:sub>2<\/jats:sub> expression and with interleukins 6 and 8, tumor necrosis factor\u2010\u03b1, interferon\u2010\u03b3, and matrix metalloproteinase\u20102 levels.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> Periodontal treatment results in PAR<jats:sub>1<\/jats:sub> overexpression in the GCF, and PAR<jats:sub>1<\/jats:sub> expression is associated with decreased expression of inflammatory biomarkers and inversely correlated to PAR<jats:sub>2<\/jats:sub> expression in the GCF. Therefore, the data suggest the importance of PAR<jats:sub>1<\/jats:sub> mediating the known anabolic actions of thrombin in the periodontium.<\/jats:p>","DOI":"10.1902\/jop.2014.140172","type":"journal-article","created":{"date-parts":[[2014,7,24]],"date-time":"2014-07-24T09:09:32Z","timestamp":1406192972000},"page":"1763-1769","source":"Crossref","is-referenced-by-count":8,"title":["Expression of Protease Activated Receptor\u20101 in Chronic Periodontitis"],"prefix":"10.1002","volume":"85","author":[{"given":"Henrique Aparecido","family":"Bueno da Silva","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanessa Tubero","family":"Euzebio Alves","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis Carlos","family":"Spolid\u00f3rio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o Batista","family":"C\u00e9sar 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