{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T10:34:50Z","timestamp":1789382090660,"version":"build-2803163510"},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,2,1]],"date-time":"2015-02-01T00:00:00Z","timestamp":1422748800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Cell\u2010based therapy using mesenchymal stem cells (MSCs) seems promising to obtain regeneration of dental tissues. A comparison of tissue sources, including periodontal ligament (PDL) versus pulp (P), could provide critical information to select an appropriate MSC population for designing predictable regenerative therapies. The purpose of this study is to compare the proliferation and stemness and the MSC\u2010specific and mineralized tissue\u2013specific gene expression of P\u2010MSCs and PDL\u2010MSCs.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> MSCs were obtained from PDL and P tissue of premolars (n = 3) extracted for orthodontic reasons. MSC proliferation was evaluated using a real\u2010time cell analyzer for 160 hours. Telomerase activity was evaluated by a telomeric repeat amplification protocol assay based on enzyme\u2010linked immunosorbent assay. Total RNA was isolated from the MSCs on day 3. A polymerase chain reaction (PCR) array was used to compare the expression of MSC\u2010specific genes. The expression of mineralized tissue\u2010associated genes, including Type I collagen (<jats:italic>COL I<\/jats:italic>), runt\u2010related transcription factor 2 (<jats:italic>RunX2<\/jats:italic>), bone sialoprotein (<jats:italic>BSP<\/jats:italic>), and osteocalcin (<jats:italic>OCN<\/jats:italic>) messenger RNA (mRNA), was evaluated using quantitative real\u2010time PCR.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Higher proliferation potential and telomerase activity were observed in the P\u2010MSCs compared to PDL\u2010MSCs of premolar teeth. Fourteen of 84 genes related to MSCs were expressed differently in the PDL\u2010MSCs versus the P\u2010MSCs. The expressions of bone morphogenetic protein 2 (<jats:italic>BMP2<\/jats:italic>) and <jats:italic>BMP6<\/jats:italic>; sex\u2010determining region Y\u2010box 9 (SOX9); integrin, alpha 6 (ITGA6); melanoma cell adhesion molecule (<jats:italic>MCAM<\/jats:italic>); phosphatidylinositol glycan anchor biosynthesis, class S (<jats:italic>PIGS<\/jats:italic>); prominin 1 (<jats:italic>PROM1<\/jats:italic>); ribosomal protein L13A (<jats:italic>RPL13A<\/jats:italic>); and microphthalmia\u2010associated transcription factor (<jats:italic>MITF<\/jats:italic>) were higher in the P\u2010MSCs compared to the PDL\u2010MSCs, and higher expression of matrix metalloproteinase 2 (<jats:italic>MMP2<\/jats:italic>), interleukin (<jats:italic>IL<\/jats:italic>)\u20106, insulin (<jats:italic>INS<\/jats:italic>), alanyl (membrane) aminopeptidase (<jats:italic>ANPEP<\/jats:italic>), and <jats:italic>IL\u201010<\/jats:italic> were observed in the PDL\u2010MSCs. However, there was no statistically significant difference in the expression of mineralized tissue\u2013associated genes, including <jats:italic>BSP<\/jats:italic> and <jats:italic>RunX2<\/jats:italic>, between the P\u2010MSCs and the PDL\u2010MSCs. Higher expression of <jats:italic>COL I<\/jats:italic> and lower expression of <jats:italic>OCN<\/jats:italic> mRNA transcripts were noted in the PDL\u2010MSCs compared to the P\u2010MSCs.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> The results of this study suggest that MSCs isolated from P and PDL tissues show different cellular behavior. To increase the predictability of MSC\u2010based regenerative treatment, differences in dental tissue\u2013derived MSCs and favorable aspects of cell sources should be further clarified.<\/jats:p>","DOI":"10.1902\/jop.2014.140257","type":"journal-article","created":{"date-parts":[[2014,10,17]],"date-time":"2014-10-17T12:52:35Z","timestamp":1413550355000},"page":"283-291","source":"Crossref","is-referenced-by-count":49,"title":["Comparison of Mesenchymal Stem Cells Isolated From Pulp and Periodontal Ligament"],"prefix":"10.1002","volume":"86","author":[{"given":"Sema S.","family":"Hakki","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seyit Ali","family":"Kayis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erdogan E.","family":"Hakki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. 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