{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T01:57:30Z","timestamp":1790474250973,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,2,1]],"date-time":"2015-02-01T00:00:00Z","timestamp":1422748800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["21792134"],"award-info":[{"award-number":["21792134"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["24593134"],"award-info":[{"award-number":["24593134"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Interleukin (IL)\u201035 plays an important role in immune regulation through the suppression of effector T\u2010cell populations, including T\u2010helper 17 (Th17) cells. Although Th17 cells and IL\u201017 are involved in the pathogenesis of periodontitis, the level of IL\u201035 in inflamed periodontal tissues is unclear. Here, IL\u201035, IL\u201017, and IL\u201027 production\/expression in gingival crevicular fluid (GCF) and human gingival tissue were investigated.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> GCF samples were collected from buccal (mesial, center, and distal) sites of teeth from patients with chronic periodontitis (CP) and healthy controls and were analyzed by enzyme\u2010linked immunosorbent assay for IL\u201035 (periodontitis, n = 36; healthy, n = 30) and IL\u201017 (periodontitis, n = 16; healthy, n = 13). Gingival tissue, including sulcus\/pocket epithelium and underlying connective tissue, was collected from an additional 10 healthy participants and 10 patients with CP and were analyzed by quantitative polymerase chain reaction (qPCR) for Epstein Barr virus\u2010induced gene 3 (<jats:italic>EBI3<\/jats:italic>), <jats:italic>IL12A<\/jats:italic>, and <jats:italic>IL17A. IL27p28<\/jats:italic> was also tested by qPCR.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> IL\u201035 and IL\u201017 were significantly higher in GCF from patients with periodontitis than healthy participants (<jats:italic>P<\/jats:italic> &lt;0.01, <jats:italic>P<\/jats:italic> &lt;0.05, respectively). In both healthy participants and those with periodontitis, positive correlations were found among IL\u201035 and probing depth and clinical attachment level (CAL) as well as between IL\u201017 and CAL. <jats:italic>EBI3<\/jats:italic>, <jats:italic>IL12A<\/jats:italic> (components of IL\u201035), and <jats:italic>IL17A<\/jats:italic> messenger RNA expression levels were significantly higher in inflamed gingival tissue than in healthy control tissues (<jats:italic>P<\/jats:italic> &lt;0.05). <jats:italic>IL27p28<\/jats:italic> was not detected in any sample, suggesting that IL\u201027 is not produced in large quantities in periodontal tissue.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> IL\u201035 and IL\u201017, but not IL\u201027, may play important roles in the pathogenesis of periodontitis.<\/jats:p>","DOI":"10.1902\/jop.2014.140293","type":"journal-article","created":{"date-parts":[[2014,10,2]],"date-time":"2014-10-02T06:11:27Z","timestamp":1412230287000},"page":"301-309","source":"Crossref","is-referenced-by-count":68,"title":["Increased Expression of Interleukin (IL)\u201035 and IL\u201017, But Not IL\u201027, in Gingival Tissues With Chronic Periodontitis"],"prefix":"10.1002","volume":"86","author":[{"given":"Akio","family":"Mitani","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanda","family":"Niedbala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeki","family":"Fujimura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makio","family":"Mogi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shin","family":"Miyamae","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoya","family":"Higuchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsushi","family":"Abe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshimitsu","family":"Hishikawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Mizutani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuichi","family":"Ishihara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Nakamura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenichi","family":"Kurita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norikazu","family":"Ohno","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshinobu","family":"Tanaka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masami","family":"Hattori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshihide","family":"Noguchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)67728-8"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345000790101301"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.153.5.2302"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxn071"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1992.63.4s.338"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345970760120501"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2260"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910808700908"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1202339"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12118"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2005.00684.x"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2011.01712.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2005.00676.x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-09-010116"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2004.08.018"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature01355"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1100667108"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034510376402"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.22.12041"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200737810"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06306"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2227"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2366"},{"key":"e_1_2_7_25_1","doi-asserted-by":"crossref","unstructured":"WhiteheadGS WilsonRH NakanoK BurchLH NakanoH CookDN. IL\u201035 production by inducible costimulator (ICOS)\u2010positive regulatory T cells reverses established IL\u201017\u2010dependent allergic airways disease.J Allergy Clin Immunol2012;129:207\u2010215.e1\u20105.","DOI":"10.1016\/j.jaci.2011.08.009"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1993.tb00348.x"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910408300216"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2013.120595"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1999.70.6.567"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1997.tb00568.x"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2014.04.007"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1999.tb02226.x"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2008.45"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0033628"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(02)00324-2"},{"key":"e_1_2_7_36_1","doi-asserted-by":"crossref","unstructured":"StumhoferJS HunterCA. Advances in understanding the anti\u2010inflammatory properties of IL\u201027.Immunol Lett2008;117:123\u2010130.","DOI":"10.1016\/j.imlet.2008.01.011"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2011.08.003"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2011.11.003"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.21342"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.20001"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2007.09.011"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200105)44:5<1003::AID-ANR179>3.0.CO;2-#"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910708600409"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2009.01390.x"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0902739"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1991.62.5.322"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jamda.2013.05.009"}],"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2014.140293","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T08:41:37Z","timestamp":1760863297000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2014.140293"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,2]]},"references-count":46,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2015,2]]}},"alternative-id":["10.1902\/jop.2014.140293"],"URL":"https:\/\/doi.org\/10.1902\/jop.2014.140293","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,2]]}}}