{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:37:13Z","timestamp":1790696233557,"version":"4.1.0"},"reference-count":52,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2015,8,1]],"date-time":"2015-08-01T00:00:00Z","timestamp":1438387200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:p><jats:bold>Background<\/jats:bold>: Nitrite is a biologic factor relevant to oral and systemic homeostasis. Through an oral bacteria reduction process, it was suggested that periodontal therapy and chlorhexidine (CHX) rinse could affect nitrite levels, leading to negative effects, such as an increase in blood pressure. This 6\u2010month randomized clinical trial evaluated the effects of periodontal therapeutic protocols on salivary nitrite and its relation to subgingival bacteria.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> One hundred patients with periodontitis were allocated randomly to debridement procedures in four weekly sections (quadrant scaling [QS]) or within 24 hours (full\u2010mouth scaling [FMS]) in conjunction with a 60\u2010day CHX (QS + CHX and FMS + CHX), placebo (QS + placebo and FMS + placebo), or no mouthrinse (QS + none and FMS + none) use. Real\u2010time polymerase chain reaction determined total bacterial, <jats:italic>Aggregatibacter actinomycetemcomitans<\/jats:italic>, <jats:italic>Porphyromonas gingivalis<\/jats:italic>, <jats:italic>Tannerella forsythia<\/jats:italic>, <jats:italic>Treponema denticola<\/jats:italic>, <jats:italic>Streptococcus oralis<\/jats:italic>, and <jats:italic>Actinomyces naeslundii<\/jats:italic> levels. Salivary nitrite concentration was determined with Griess reagent. Data were analyzed statistically at baseline and 3 and 6 months by analysis of variance, Kruskal\u2013Wallis, Mann\u2013Whitney <jats:italic>U<\/jats:italic>, and Spearman correlation tests (<jats:italic>P<\/jats:italic> &lt;0.05).<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Nitrite concentrations did not tend to change over time. Regarding CHX use, there was a negative correlation between nitrite and total bacterial load at 6 months (FMS + CHX) and one positive correlation between <jats:italic>P. gingivalis<\/jats:italic> and nitrite at baseline (QS + CHX). Independently of rinse type, in the FMS group, nitrite correlated negatively with several microbial parameters and also with a higher percentage of deep periodontal pockets.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> The relationship between nitrite and bacterial levels appears weak. Short\u2010term scaling exhibited a greater influence on nitrite concentrations then long\u2010term CHX use.<\/jats:p>","DOI":"10.1902\/jop.2015.140678","type":"journal-article","created":{"date-parts":[[2015,3,26]],"date-time":"2015-03-26T09:45:39Z","timestamp":1427363139000},"page":"984-994","source":"Crossref","is-referenced-by-count":24,"title":["Periodontal Therapy Effects on Nitrite Related to Oral Bacteria: A 6\u2010Month Randomized Clinical Trial"],"prefix":"10.1002","volume":"86","author":[{"given":"Sheila C.","family":"Cortelli","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando O.","family":"Costa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edson","family":"Rodrigues","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis 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