{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T18:36:34Z","timestamp":1776796594182,"version":"3.51.2"},"reference-count":36,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2015,11,1]],"date-time":"2015-11-01T00:00:00Z","timestamp":1446336000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Many invading oral bacteria are known to produce considerable amounts of hydrogen sulfide (H<jats:sub>2<\/jats:sub>S). The toxic activity of exogenous H<jats:sub>2<\/jats:sub>S in periodontal tissue has been demonstrated, but the role of endogenous H<jats:sub>2<\/jats:sub>S in the physiologic function of periodontal tissue remains poorly understood. The purpose of the present study is to investigate the biologic functions of H<jats:sub>2<\/jats:sub>S in the proliferation and differentiation of human periodontal ligament stem cells (PDLSCs).<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> PDLSCs were isolated from periodontal ligament tissues of periodontally healthy volunteers or patients with periodontitis. Immunocytochemical staining, flow cytometry, and Western blot analysis were used to examine the expression of H<jats:sub>2<\/jats:sub>S\u2010synthesizing enzymes cystathionine\u2010\u03b2\u2010synthase (CBS) and cystathionine\u2010\u03b3\u2010lyase (CSE). The proliferation capacity of PDLSCs was determined by cell counting kit\u20108 assay, carboxyfluorescein succinimidyl ester analysis, and 5\u2010ethynyl\u20102\u2032\u2010deoxyuridine assay. The osteogenic potential of PDLSCs was tested using alkaline phosphatase staining, Alizarin Red staining, and in vivo transplantation experiments. Oil Red O staining was used to analyze adipogenic ability.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> The results show that human PDLSCs express both CBS and CSE and produce H<jats:sub>2<\/jats:sub>S. Blocking the generation of endogenous H<jats:sub>2<\/jats:sub>S with CBS inhibitor hydroxylamine significantly attenuated PDLSC proliferation and reduced the osteogenic and adipogenic differentiation capacity of PDLSCs. In contrast, CSE inhibitor <jats:sc>dl<\/jats:sc>\u2010propargylglycine had no effect on PDLSC function. Exogenous H<jats:sub>2<\/jats:sub>S could inhibit the production of endogenous H<jats:sub>2<\/jats:sub>S and impair PDLSC function in a dose\u2010dependent manner.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> Physiologic levels of endogenous H<jats:sub>2<\/jats:sub>S maintain the proliferation and differentiation capacity of PDLSCs, and CBS may be the main source of endogenous H<jats:sub>2<\/jats:sub>S in PDLSCs.<\/jats:p>","DOI":"10.1902\/jop.2015.150240","type":"journal-article","created":{"date-parts":[[2015,8,13]],"date-time":"2015-08-13T11:33:45Z","timestamp":1439465625000},"page":"1276-1286","source":"Crossref","is-referenced-by-count":37,"title":["Physiologic Levels of Endogenous Hydrogen Sulfide Maintain the Proliferation and Differentiation Capacity of Periodontal Ligament Stem 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