{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T07:29:23Z","timestamp":1791962963911,"version":"4.3.4"},"reference-count":50,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,6,1]],"date-time":"2016-06-01T00:00:00Z","timestamp":1464739200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002923","name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","doi-asserted-by":"publisher","award":["PIP 2012\u20102014 076"],"award-info":[{"award-number":["PIP 2012\u20102014 076"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2016,6]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Anti\u2010inflammatory and immunologic properties of cannabinoids have been reported in several tissues. Expression of cannabinoid receptor Type 2 was reported in osteoblasts and osteoclasts, suggesting a key role in bone metabolism. The aim of this study is to assess the effect of treatment with cannabinoid\u20102 receptor agonist HU\u2010308 in the oral health of rats subjected to lipopolysaccharide (LPS)\u2010induced periodontitis.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Twenty\u2010four rats were distributed in four groups (six rats per group): 1) control rats; 2) sham rats; 3) rats submitted to experimental periodontitis (LPS); and 4) rats submitted to experimental periodontitis and treated with HU\u2010308 (LPS+HU). In groups LPS and LPS+HU, periodontitis was induced by LPS (1 mg\/mL) injected into the gingival tissue (GT) of maxillary and mandibular first molars and into the interdental space between the first and second molars, 3 days per week for 6 weeks. In group LPS+HU, HU\u2010308 (500 ng\/mL) was applied topically to the GT daily.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Alveolar bone loss resulting from LPS\u2010induced periodontitis was significantly attenuated with HU\u2010308 treatment (LPS+HU), measured by macroscopic and histologic examination. Treatment also reduced gingival production of inflammatory mediators augmented in LPS\u2010injected rats, such as: 1) inducible nitric oxide (iNOS) activity (LPS: 90.18 \u00b1 36.51 pmol\/minute\/mg protein versus LPS+HU: 16.37 \u00b1 4.73 pmol\/minute\/mg protein; <jats:italic>P<\/jats:italic> &lt;0.05); 2) tumor necrosis factor alpha (LPS: 185.70 \u00b1 25.63 pg\/mg protein versus LPS+HU: 95.89 \u00b1 17.47 pg\/mg protein; <jats:italic>P<\/jats:italic> &lt;0.05); and 3) prostaglandin E<jats:sub>2<\/jats:sub> (PGE<jats:sub>2<\/jats:sub>) (LPS: 159.20 \u00b1 38.70 pg\/mg wet weight versus LPS+HU: 71.25 \u00b1 17.75 pg\/mg wet weight; <jats:italic>P<\/jats:italic> &lt;0.05). Additionally, HU\u2010308 treatment prevented the inhibitory effect of LPS\u2010induced periodontitis on the salivary secretory response to pilocarpine. Moreover, iNOS activity and PGE<jats:sub>2<\/jats:sub> content, which were increased by LPS\u2010induced periodontitis in the submandibular gland, returned to control values after HU\u2010308 treatment.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> This study demonstrates anti\u2010inflammatory, osteoprotective, and prohomeostatic effects of HU\u2010308 in oral tissues of rats with LPS\u2010induced periodontitis.<\/jats:p>","DOI":"10.1902\/jop.2016.150612","type":"journal-article","created":{"date-parts":[[2016,2,5]],"date-time":"2016-02-05T03:23:10Z","timestamp":1454642590000},"page":"725-734","source":"Crossref","is-referenced-by-count":57,"title":["Anti\u2010Inflammatory and Osteoprotective Effects of Cannabinoid\u20102 Receptor Agonist HU\u2010308 in a Rat Model of Lipopolysaccharide\u2010Induced Periodontitis"],"prefix":"10.1002","volume":"87","author":[{"given":"Cesar 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