{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T15:31:01Z","timestamp":1784561461409,"version":"3.55.0"},"reference-count":37,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T00:00:00Z","timestamp":1472688000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Periodontal regeneration of incisors is necessary for esthetic recovery. A novel regenerative method combining bone morphogenetic protein (BMP)\u20102 and fibroblast growth factor (FGF)\u20102 was developed. The purpose of this study is to evaluate periodontal healing, including root coverage, in circumferential defects of incisors.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Fifty incisors in five beagles were used. After circumferential defects were surgically created, each group, consisting of ten recipient sites, received: 1) a double layer with FGF\u20102 (2 \u03bcg)\/collagen as inner layer and BMP\u20102 (4 \u03bcg)\/collagen as outer layer (FB\u2010DL group); 2) collagen impregnated with both FGF\u20102 (2 \u03bcg) and BMP\u20102 (4 \u03bcg) (FB\u2010M group); 3) BMP\u20102 (4 \u03bcg)\/collagen (B group); 4) FGF\u20102 (4 \u03bcg)\/collagen (F group); or 5) collagen (C group). Dogs were sacrificed 8 weeks post\u2010surgery, and healing was evaluated histologically.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> The three groups treated with BMP\u20102 showed enhanced new bone formation compared with control and F groups (<jats:italic>P<\/jats:italic> &lt; 0.05). Furthermore, connective tissue attachment with cementum regeneration in the FB\u2010DL group was significantly greater than in FB\u2010M and B groups (<jats:italic>P<\/jats:italic> &lt;0.05). Ankylosis in the FB\u2010DL group was significantly less than in FB\u2010M and B groups (<jats:italic>P<\/jats:italic> &lt;0.05). Gingival recession was inhibited significantly better in FB\u2010DL and FB\u2010M groups compared with control and B groups.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> These data support development of a double\u2010layer method combining BMP\u20102 and FGF\u20102 as a therapeutic approach to periodontal regeneration at incisors with horizontal circumferential defects.<\/jats:p>","DOI":"10.1902\/jop.2016.150746","type":"journal-article","created":{"date-parts":[[2016,5,31]],"date-time":"2016-05-31T04:15:54Z","timestamp":1464668154000},"page":"1067-1074","source":"Crossref","is-referenced-by-count":7,"title":["A Novel Regenerative Technique Combining Bone Morphogenetic Protein\u20102 With Fibroblast Growth Factor\u20102 for Circumferential Defects in Dog 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