{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T20:28:08Z","timestamp":1768768088470,"version":"3.49.0"},"reference-count":47,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2017,4,1]],"date-time":"2017-04-01T00:00:00Z","timestamp":1491004800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> The collection of subgingival plaque samples with paper points is time\u2010consuming and accident\u2010sensitive. However, the collection of saliva is simple and contains pathogens of all intraoral surfaces. The aim of this study is to investigate whether a sampling strategy with mouthrinse (mouthrinse sample [MSP]; test) leads to results comparable with standard sampling method (multiple site test from the deepest pocket of each quadrant [MT4]; control).<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> In 50 patients with periodontitis, subgingival plaque was sampled from the deepest pocket of each quadrant by using paper points and by gaining saliva with saline mouthrinse. Analysis was performed using a commercially available polymerase chain reaction test for 11 periodontal pathogens.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Detection frequency of <jats:italic>Aggregatibacter actinomycetemcomitans<\/jats:italic> (MT4\/MSP: 42%\/36%), <jats:italic>Porphyromonas gingivalis<\/jats:italic> (78%\/66%), <jats:italic>Tannerella forsythia<\/jats:italic> (98%\/84%), <jats:italic>Treponema denticola<\/jats:italic> (94%\/74%), <jats:italic>Parvimonas micra<\/jats:italic> (86%\/62%), <jats:italic>Campylobacter rectus<\/jats:italic> (90%\/76%), <jats:italic>Eubacterium nodatum<\/jats:italic> (64%\/30%), <jats:italic>Prevotella intermedia<\/jats:italic> (58%\/54%), and <jats:italic>Eikenella corrodens<\/jats:italic> (90%\/82%) was higher with MT4 than MSP. For <jats:italic>Fusobacterium nucleatum<\/jats:italic> (100%\/100%), there was no difference between test and control. Only detection frequency of <jats:italic>Capnocytophaga<\/jats:italic> species (68%\/74%) was higher with MSP than MT4. Differences were significant for <jats:italic>P. gingivalis<\/jats:italic>, <jats:italic>T. forsythia<\/jats:italic>, <jats:italic>T. denticola<\/jats:italic>, <jats:italic>P. micra<\/jats:italic>, <jats:italic>C. rectus<\/jats:italic>, and <jats:italic>E. nodatum<\/jats:italic>.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> There is no significant difference between MT4 and MSP for detection frequency of key pathogen <jats:italic>A. actinomycetemcomitans<\/jats:italic>. Key pathogens <jats:italic>P. gingivalis<\/jats:italic>, <jats:italic>T. forsythia<\/jats:italic>, <jats:italic>T. denticola<\/jats:italic>, <jats:italic>P. micra<\/jats:italic>, <jats:italic>C. rectus<\/jats:italic>, and <jats:italic>E. nodatum<\/jats:italic> show statistically higher detection frequencies with MT4.<\/jats:p>","DOI":"10.1902\/jop.2016.160249","type":"journal-article","created":{"date-parts":[[2016,10,27]],"date-time":"2016-10-27T11:29:33Z","timestamp":1477567773000},"page":"399-406","source":"Crossref","is-referenced-by-count":28,"title":["Comparison of Two Different Sampling Methods for Subgingival Plaque: Subgingival Paper Points or Mouthrinse Sample?"],"prefix":"10.1002","volume":"88","author":[{"given":"Katrin","family":"Nickles","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susanne","family":"Scharf","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lasse","family":"R\u00f6llke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bettina","family":"Dannewitz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Eickholz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,4]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1998.tb02419.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051X.2002.291107.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2012.11.006"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1987.tb00949.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1976.47.7.373"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1999.4.1.39"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2007.01080.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(08)60135-X"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2005.00805.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1532-3382(12)70019-2"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1993.64.4.243"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1992.63.5.418"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1985.56.8.443"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1993.tb00397.x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1994.65.9.820"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1991.tb02067.x"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1999.70.6.574"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2005.76.5.749"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.1996.tb00068.x"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12441"},{"key":"e_1_2_7_22_1","first-page":"660","article-title":"Microbiological diagnostics in the therapy of periodontitis: Joint statement of the German Society of Periodontology (DGP) and the German Society of Dental, Oral, and Maxillofacial Medicine (DGZMK) (in German)","volume":"60","author":"Beikler T","year":"2005","journal-title":"Dtsch Zahnarztl Z"},{"issue":"5","key":"e_1_2_7_23_1","first-page":"867","article-title":"Parameter on aggressive periodontitis","volume":"71","author":"American Academy of Periodontology","year":"2000","journal-title":"J Periodontol"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2001.72.12.1790"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2041-1626.2010.00026.x"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-015-1530-4"},{"key":"e_1_2_7_27_1","unstructured":"Federal Office of Consumer Protection and Food Safety Paul\u2010Ehrlich\u2010Gesellschaft f\u00fcr Chemotherapie e.V. Infectology Freiburg. GERMAP 2012 \u2013Report on the consumption of antimicrobials and the spread of antimicrobial resistance in human and veterinary medicine in Germany. Rheinbach: Antiinfectives Intelligence; 2014."},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2007.060236"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2006.050204"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1991.62.3.203"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1998.69.7.828"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2005.76.2.204"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2007.060078"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-011-0654-4"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2013.130306"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1989.tb01626.x"},{"key":"e_1_2_7_37_1","first-page":"153","article-title":"Clinical and microbiological effects of a combined mechanic\u2010antibiotic therapy in subjects with Actinobacillus actinomycetemcomitans\u2010associated periodontitis","volume":"20","author":"Dannewitz B","year":"2007","journal-title":"Am J Dent"},{"key":"e_1_2_7_38_1","first-page":"235","article-title":"Antibiotics in periodontal therapy","volume":"2","author":"Eickholz P","year":"2005","journal-title":"Perio"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-014-1208-3"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051X.2002.290708.x"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2010.08.017"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2009.01434.x"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-009-0333-x"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-302X.1999.140510.x"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.2307\/2529310"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.084616.108"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1902\/annals.2003.8.1.182"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2010.01679.x"}],"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2016.160249","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T09:42:59Z","timestamp":1760866979000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2016.160249"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,4]]},"references-count":47,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2017,4]]}},"alternative-id":["10.1902\/jop.2016.160249"],"URL":"https:\/\/doi.org\/10.1902\/jop.2016.160249","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,4]]}}}