{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T01:05:36Z","timestamp":1785200736795,"version":"3.55.0"},"reference-count":50,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2017,7,1]],"date-time":"2017-07-01T00:00:00Z","timestamp":1498867200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002873","name":"Chulalongkorn University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002873","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Increased level of proinflammatory cytokine interleukin (IL)\u201012 correlates with the severity of periodontitis. Yet, a possible role of IL\u201012 in periodontal disease has not been clarified. The aim of this study is to investigate whether IL\u201012 affects expression of receptor activator of nuclear factor\u2010kappa B (NF\u2010\u03baB) ligand (RANKL), a potent osteoclast\u2010stimulating factor, by human periodontal ligament (hPDL) cells.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> To determine the effect of IL\u201012, hPDL cells were incubated with recombinant human IL\u201012 (p70) in a dose\u2010 (0 to 10 ng\/mL) and time\u2010dependent manner. Expression of RANKL was evaluated at mRNA and protein levels. Underlying signaling pathways of IL\u201012 were determined by using specific inhibitors.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Under the influence of IL\u201012, hPDL cells expressed significantly higher levels of RANKL. Expression was mediated by signal transducer and activator of transcription 4 and NF\u2010\u03baB signaling pathways. Conditioned medium of IL\u201012\u2013incubated cells proved to contain molecule(s) that induced RANKL expression. Addition of suramin (G protein\u2013coupled receptor inhibitor) and ethylene glycol tetraacetic acid (calcium chelator) suggested existence of intermediate molecule(s) that could activate heterotrimeric G protein signaling in a calcium\u2010dependent pathway.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> Expression of RANKL by hPDL cells significantly increased after IL\u201012 treatment. Therefore, this study supports a close interrelationship between immune and skeletal systems and suggests an osteolytic role of IL\u201012 in pathogenesis of periodontal disease.<\/jats:p>","DOI":"10.1902\/jop.2017.160813","type":"journal-article","created":{"date-parts":[[2017,3,3]],"date-time":"2017-03-03T10:49:23Z","timestamp":1488538163000},"source":"Crossref","is-referenced-by-count":11,"title":["Interleukin\u201012 Induces Receptor Activator of Nuclear Factor\u2010Kappa B Ligand Expression by Human Periodontal Ligament Cells"],"prefix":"10.1002","volume":"88","author":[{"given":"Benjar","family":"Issaranggun Na Ayuthaya","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Everts","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prasit","family":"Pavasant","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12002"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2011.01.034"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1994.65.7.641"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.128.5.2177"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.2.744-750.2001"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.1998.tb00255.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3002"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12063"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2005-0008"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12421"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.177.4.1199"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2004.04.081"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)00045-5"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9969(98)00094-6"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/13.8.1053"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.12.6495"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1155\/2010\/327417"},{"key":"e_1_2_7_19_1","first-page":"147","article-title":"Serum levels of TNF\u2010alpha, TNF\u2010alphaRI, TNF\u2010alphaRII and IL\u201012 in treated rheumatoid arthritis patients","volume":"6","author":"Ebrahimi AA","year":"2009","journal-title":"Iran J Immunol"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1177\/10454411980090040701"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2011.01798.x"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2005.02.007"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.9.6193"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2009.217"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2062"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.20.100301.064753"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1700451"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1006\/mcbr.2000.0205"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1359\/jbmr.2002.17.2.210"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00223-001-2086-7"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.34332"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.180.1.223"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1359-6101(03)00043-1"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80614-7"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0143-4160(98)90060-4"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.72.5.995"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63016-7"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.2006.00185.x"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2004.09.011"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/35046102"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI30074"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2009.12.030"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0105-2896.2006.00375.x"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1002628"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01116857"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4889(03)00089-2"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)47340-7"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.7.2466"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1993.1817"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1993.264.3.C519"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/0143-4160(92)90065-Z"}],"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2017.160813","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T12:57:38Z","timestamp":1760878658000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2017.160813"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7]]},"references-count":50,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2017,7]]}},"alternative-id":["10.1902\/jop.2017.160813"],"URL":"https:\/\/doi.org\/10.1902\/jop.2017.160813","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,7]]}}}