{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T23:01:18Z","timestamp":1785366078586,"version":"3.55.0"},"reference-count":49,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2017,8,1]],"date-time":"2017-08-01T00:00:00Z","timestamp":1501545600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81100761"],"award-info":[{"award-number":["81100761"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81171470"],"award-info":[{"award-number":["81171470"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Osteoporosis is associated with widespread periodontitis and impaired periodontal healing. However, there is a lack of information about the outcomes of regenerative approaches under the influence of osteoporosis. This study investigates the effect of leptin (LEP) overexpression on the regenerative potential of bone marrow stromal cells (BMSCs) in an osteoporotic rat periodontal fenestration defect model.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Rat BMSCs were transfected with adenoviruses harboring the human <jats:italic>(h)LEP<\/jats:italic> gene. Cell proliferation and osteogenic differentiation were evaluated. A \u03b2\u2010tricalcium phosphate scaffold seeded with transfected cells was implanted into nude mice to investigate ectopic osteogenesis and into an osteoporotic rat defect to study periodontal regeneration. Regenerated periodontal and bone\u2010like tissues were analyzed by histologic methods.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> <jats:italic>hLEP<\/jats:italic> overexpression induced osteogenic differentiation of BMSCs as evidenced by the upregulation of osteogenesis\u2010related genes such as Runt\u2010related transcription factor 2, alkaline phosphatase (<jats:italic>ALP<\/jats:italic>), and collagen Type I, as well as increased <jats:italic>ALP<\/jats:italic> activity and enhanced mineralization. Mice implanted with <jats:italic>hLEP<\/jats:italic>\u2010BMSC\u2013containing scaffolds showed more extensive formation of bone\u2010like tissue than those in other groups. Periodontal defects were also filled to a greater degree when treated with <jats:italic>hLEP<\/jats:italic>\u2010BMSCs and contained cementum and a well\u2010organized periodontal ligament after 10 and 28 days.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> <jats:italic>hLEP<\/jats:italic> overexpression in BMSCs can stimulate periodontal regeneration in osteoporotic conditions and might be a promising strategy for periodontal regeneration in patients with osteoporosis.<\/jats:p>","DOI":"10.1902\/jop.2017.170042","type":"journal-article","created":{"date-parts":[[2017,4,25]],"date-time":"2017-04-25T09:39:57Z","timestamp":1493113197000},"page":"808-818","source":"Crossref","is-referenced-by-count":34,"title":["Leptin Overexpression in Bone Marrow Stromal Cells Promotes Periodontal Regeneration in a Rat Model of Osteoporosis"],"prefix":"10.1002","volume":"88","author":[{"given":"Baoyu","family":"Zheng","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuling","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minkui","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhibin","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yin","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuhua","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep22005"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cden.2005.03.003"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.3109\/13697137.2010.500749"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1254\/jphs.11188SC"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1538\/expanim.61.183"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1079-2104(05)80158-1"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2011.01881.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2006.00902.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2005.01.002"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.2012.00443.x"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jor.1100090504"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar614"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1582-4934.2004.tb00320.x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.22203\/eCM.v026a19"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/eos.12122"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2011.01820.x"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2012.08.011"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature03398"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.20330"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-015-2167-y"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1177\/00220345970760080801"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.msec.2016.08.045"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-015-0023-3"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60611-2"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2008.114"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2010.06.026"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12379"},{"key":"e_1_2_7_29_1","first-page":"841","article-title":"Overexpression of integrin a2 promotes osteogenic differentiation of hBMSCs from senile osteoporosis through the ERK pathway","volume":"6","author":"Hu HM","year":"2013","journal-title":"Int J Clin Exp Pathol"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00198-013-2494-3"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0045142"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.10.3838"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0501.2003.110770.x"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12354"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1177\/0022034513518561"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2012.01512.x"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2015.120448"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0131870"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1210\/endo.140.4.6637"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1750405"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.10156"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10616-010-9284-y"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2008.02.030"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2010.00998.x"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2004.00752.x"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.3109\/14653241003709702"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1530\/rep.1.00557"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.2018"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-860-3_13"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/jre.12111"}],"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.2017.170042","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T08:51:04Z","timestamp":1760863864000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.2017.170042"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8]]},"references-count":49,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2017,8]]}},"alternative-id":["10.1902\/jop.2017.170042"],"URL":"https:\/\/doi.org\/10.1902\/jop.2017.170042","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8]]}}}