{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T20:41:48Z","timestamp":1788381708684,"version":"build-2803163510"},"reference-count":49,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,2,6]],"date-time":"2018-02-06T00:00:00Z","timestamp":1517875200000},"content-version":"vor","delay-in-days":36,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005283","name":"Funda\u00e7\u00e3o Cearense de Apoio ao Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005283","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["23038.009502\/2013"],"award-info":[{"award-number":["23038.009502\/2013"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2015\/23701\u20100"],"award-info":[{"award-number":["2015\/23701\u20100"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Tiludronic acid (TIL) presents antiresorptive and anti\u2010inflammatory properties and has not been evaluated in the periodontitis\u2013diabetes mellitus (DM) association to date, to the best knowledge of the authors. This study evaluates effects of local administration of TIL on experimental periodontitis (EP) in rats with streptozotocin\u2010induced DM.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Thirty\u2010two animals (<jats:italic>Rattus norvegicus albinus<\/jats:italic>, Wistar) were divided into groups DM\/C (Control), DM\/EP, DM\/EP\/TIL1, and DM\/EP\/TIL3. In EP groups, a ligature was placed around mandibular first molars. In groups DM\/EP\/TIL1 and DM\/EP\/TIL3, TIL solutions (1 and 3\u00a0mg\/kg, respectively) were injected into the gingival tissue of mandibular molars every other day for 10 days, until euthanasia. Periodontal tissues were analyzed by microcomputed tomography (micro\u2010CT), histomorphometry, immunohistochemistry (tartrate\u2010resistant acid phosphatase [TRAP], receptor activator of nuclear factor\u2010\u03baB ligand [RANKL], osteoprotegerin, cleaved caspase 3), and quantitative reverse transcription\u2010polymerase chain reaction (interleukin [IL]\u20101\u03b2, vascular endothelial growth factor [VEGF]).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In micro\u2010CT analyses, groups DM\/EP\/TIL1 and DM\/EP\/TIL3 presented reduced alveolar bone resorption (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05). Group DM\/EP\/TIL3 presented decreased attachment loss (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05). The amount of TRAP\u2010positive multinucleated cells was decreased in TIL groups (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05). Group DM\/EP\/TIL3 presented a lower immunolabeling pattern for RANKL (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05). TIL treatment decreased genic expression of IL\u20101\u03b2, and in group DM\/EP\/TIL3, expression of VEGF was increased (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Local administration of TIL promoted a protective effect against tissue destruction in EP in diabetic rats, and the dosage of 3\u00a0mg\/kg of TIL promoted the best results regarding its antiresorptive and anti\u2010inflammatory effects.<\/jats:p><\/jats:sec>","DOI":"10.1902\/jop.2017.170228","type":"journal-article","created":{"date-parts":[[2017,9,15]],"date-time":"2017-09-15T10:24:22Z","timestamp":1505471062000},"page":"105-116","source":"Crossref","is-referenced-by-count":7,"title":["Effects of local administration of tiludronic acid on experimental periodontitis in diabetic rats"],"prefix":"10.1002","volume":"89","author":[{"given":"Nara L.T.","family":"Nunes","sequence":"first","affiliation":[{"name":"Department of Clinical Dentistry Graduate Program in Dentistry Faculty of Pharmacy Dentistry and Nursing Federal University of Cear\u00e1  Fortaleza Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel R.","family":"Messora","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery and Periodontology School of Dentistry of Ribeirao Preto University of S\u00e3o Paulo, Ribeirao Preto  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz Fernando","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery and Periodontology School of Dentistry of Ribeirao Preto University of S\u00e3o Paulo, Ribeirao Preto  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Lisboa","sequence":"additional","affiliation":[{"name":"Department of Morphology Faculty of Medicine Federal University of Cear\u00e1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina Costa Barcellos","family":"Garcia","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery and Periodontology School of Dentistry of Ribeirao Preto University of S\u00e3o Paulo, Ribeirao Preto  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.O.","family":"R\u00eago","sequence":"additional","affiliation":[{"name":"Department of Dentistry School of Dentistry at Sobral Federal University of Cear\u00e1  Sobral Cear\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Arthur Bel\u00e9m","family":"Novaes","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery and Periodontology School of Dentistry of Ribeirao Preto University of S\u00e3o Paulo, Ribeirao Preto  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9rgio Lu\u00eds Scombatti","family":"de Souza","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery and Periodontology School of Dentistry of Ribeirao Preto University of S\u00e3o Paulo, Ribeirao Preto  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edilson","family":"Ervolino","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences Division of Histology Dental School of Ara\u00e7atuba S\u00e3o Paulo State University, Ara\u00e7atuba   S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fl\u00e1via A.C.","family":"Furlaneto","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery and Periodontology School of Dentistry of Ribeirao Preto University of S\u00e3o Paulo, Ribeirao Preto  S\u00e3o Paulo 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