{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T04:28:02Z","timestamp":1782707282888,"version":"3.54.5"},"reference-count":0,"publisher":"OAE Publishing Inc.","issue":"1","content-domain":{"domain":["oaepublish.com"],"crossmark-restriction":false},"short-container-title":["Extracell Vesicles Circ Nucleic Acids"],"abstract":"<jats:p>Background: Vascular cell adhesion molecule-1 (VCAM-1+) endothelial cell-derived extracellular vesicles (EC-EVs) are augmented in cardiovascular disease, where they can signal the deployment of immune cells from the splenic reserve. Endothelial cells in culture activated with pro-inflammatory tumor necrosis factor-\u03b1 (TNF-a) also release VCAM-1+ EC-EVs. However, isolating VCAM-1+ EC-EVs from conditioned cell culture media for subsequent in-depth analysis remains challenging.  Aim: We utilized the extracellular vesicles (EV) microfluidics herringbone chip (EVHB-Chip), coated with anti-VCAM-1 antibodies, for selective capture of VCAM-1+ cells and EC-EVs.  Methods and Results: Engineered EA.hy926 endothelial cells overexpressing VCAM-1 (P &lt; 0.001 versus control) showed increased binding to the VCAM-1- EVHB-Chip versus an IgG device. TNF-\u03b1-stimulated human umbilical cord vein endothelial cells (HUVECs) exhibited elevated VCAM-1 protein levels (P &lt; 0.001) and preferential binding to the VCAM-1- EVHB-Chip versus the IgG device. HUVECs stimulated with TNF-\u03b1 showed differential gene expression of intercellular adhesion molecule-1 (ICAM-1) (P &lt; 0.001) and VCAM-1 (P &lt; 0.001) by digital droplet PCR versus control cells. HUVEC-derived EC-EVs were positive for CD9, CD63, HSP70, and ALIX and had a modal size of 83.5 nm. Control and TNF-\u03b1-stimulated HUVEC-derived EC-EV cultures were captured on the VCAM-1- EVHB-Chip, demonstrating selective capture. VCAM-1+ EC-EV were significantly enriched for ICAM-1 (P &lt; 0.001) mRNA transcripts.  Conclusion: This study presents a novel approach using the EVHB-Chip, coated with anti-VCAM-1 antibodies and digital droplet PCR for the study of VCAM-1+ EC-EVs. Isolation of VCAM-1+ EC-EV from heterogeneous sources such as conditioned cell culture media holds promise for subsequent detailed characterization, and may facilitate the study of VCAM-1+ EC-EVs in cardiovascular and metabolic diseases, for disease monitoring and therapeutic insights.<\/jats:p>","DOI":"10.20517\/evcna.2023.51","type":"journal-article","created":{"date-parts":[[2024,2,8]],"date-time":"2024-02-08T02:01:40Z","timestamp":1707357700000},"page":"83-94","update-policy":"https:\/\/doi.org\/10.20517\/oae.crossmark.policy","source":"Crossref","is-referenced-by-count":4,"title":["The isolation of VCAM-1+ endothelial cell-derived extracellular vesicles using microfluidics"],"prefix":"10.20517","volume":"5","author":[{"given":"Naveed","family":"Akbar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evelyn Grace","family":"Luciani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raheel","family":"Ahmad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dasol","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Veiga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel Christopher","family":"Rabe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shannon Leigh","family":"Stott","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"8410","published-online":{"date-parts":[[2023]]},"container-title":["Extracellular Vesicles and Circulating Nucleic Acids"],"original-title":[],"deposited":{"date-parts":[[2024,2,8]],"date-time":"2024-02-08T02:01:41Z","timestamp":1707357701000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.oaepublish.com\/articles\/evcna.2023.51"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023]]},"references-count":0,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2023]]}},"URL":"https:\/\/doi.org\/10.20517\/evcna.2023.51","relation":{},"ISSN":["2767-6641"],"issn-type":[{"value":"2767-6641","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023]]}}}