{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T01:15:13Z","timestamp":1787879713566,"version":"build-2784847793"},"reference-count":37,"publisher":"OAE Publishing Inc.","issue":"2","content-domain":{"domain":["oaepublish.com"],"crossmark-restriction":false},"short-container-title":["Extracell Vesicles Circ Nucleic Acids."],"abstract":"<jats:p>Aim: Dry eye disease (DED) is a multifactorial disorder characterized by tear film instability, ocular surface damage, and inflammation. This study investigates the molecular landscape and the therapeutic potential of umbilical cord mesenchymal stem cell-derived extracellular vesicles (UCMSC-EVs) as a standardized, cell-free treatment for DED.<\/jats:p>\n                  <jats:p>Methods: UCMSC-EVs were produced from UCMSCs in a scalable 3D bioreactor system and enriched via tangential flow filtration (TFF). The physicochemical properties of UCMSC-EVs were characterized. The molecular cargo of UCMSC-EVs was analyzed via proteomics analysis and microRNA (miRNA) microarray profiling across multiple production lots. Functional potency of UCMSC-EVs was assessed using a human corneal epithelial cell line (HCE-S) wound healing assay and two human inflammation models.<\/jats:p>\n                  <jats:p>Results: UCMSC-EVs exhibited a consistent cargo profile enriched with proteins and miRNAs targeting DED-related pathways, with high proteomic correlation (Pearson\u2019s r &gt; 0.8) across independent lots. Functional assays demonstrated that UCMSC-EVs promoted HCE-S wound healing and suppressed pro-inflammatory cytokines in a dose-dependent manner. Furthermore, a significant negative correlation was identified between PEDF (Pigment Epithelium-Derived Factor) cargo concentration and interferon gamma (IFN-\u03b3) secretion (Pearson\u2019s r = -0.724, P = 0.005), providing a quantitative link between molecular identity and anti-inflammatory potency.<\/jats:p>\n                  <jats:p>Conclusion: UCMSC-EVs possess favorable physicochemical and molecular characteristics, and demonstrate robust regenerative and anti-inflammatory properties in vitro. The high manufacturing consistency and the identification of key molecular drivers, such as PEDF, support the potential of UCMSC-EV as a well-characterized and viable cell-free therapy for mitigating DED progression.<\/jats:p>","DOI":"10.20517\/evcna.2025.177","type":"journal-article","created":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T08:12:56Z","timestamp":1781597576000},"page":"927-44","update-policy":"https:\/\/doi.org\/10.20517\/oae.crossmark.policy","source":"Crossref","is-referenced-by-count":1,"title":["Molecular profiling and functional analyses of umbilical cord mesenchymal stem cell-derived extracellular vesicles for dry eye 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