{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T02:59:58Z","timestamp":1771469998849,"version":"3.50.1"},"reference-count":0,"publisher":"African Traditional Herbal Medicine Supporters Initiative (ATHMSI)","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Afr J  Tradit Complement Altern Med"],"abstract":"<jats:p>Background: Ormenis eriolepis Coss (Asteraceae) is an endemic Moroccan subspecies, traditionally named \u201cHellala\u201d\r\nor \u201cFergoga\u201d. It\u2019s usually used for its hypoglycemic effect as well as for the treatment of stomacal pain. As far as we\r\nknow, there is no scientific exploration of anti tumoral activity of Ormenis eriolepis extracts.\r\nMaterials and Methods: In this regard, we performed a screening of organic extracts and fractions in a panel of both\r\nhematological and solid cancer cell lines, to evaluate the potential in vitro anti tumoral activity and to elucidate the\r\nrespective mechanisms that may be responsible for growth arrest and cell death induction. The plant was extracted\r\nusing organic solvents, and four different extracts were screened on Jurkat, Jeko-1, TK-6, LN229, SW620, U2OS, PC-3\r\nand NIH3T3 cells.\r\nResults: Cell viability assays revealed that, the IC50 values were (11,63\u00b15,37\u03bcg\/ml) for Jurkat, (13,33\u00b11,67\u03bcg\/ml) for\r\nJeko-1, (41,67\u00b11,98\u03bcg\/ml) for LN229 and (19,31\u00b14,88\u03bcg\/ml) for PC-3 cells upon treatment with Oe-DF and Oe-HE\r\nrespectively. Both the fraction and extract exhibited no effects on TK6 and NIH3T3. Cytometry analysis accompanied\r\nby DNA damage signaling protein levels monitoring (p-H2A.X), showed that both the Dichloromethane Fraction and\r\nHexanic extract induce DNA double stranded breaks (DSBs) accompanied by cell cycle arrest in G1 (Jurkat, Jeko -1\r\nand LN22) and G2\/M (PC-3) phases which is agreed with the caspase activity observed. Additional experiments with\r\nselective inhibitors of stress and survival pathways (JNK, MAPK, Rho, p53, and JAK3) indicated that none of these\r\npathways was significantly involved in apoptosis induction. The bioactive compound analysis by CG\/MS indicated that\r\nthe major compounds in Oe-DF were: Linoleic Acid (15,89%), Podophyllotoxin (17,89%) and Quercetin (22,95%). For\r\nOe-HE the major molecules were: Linoleic Acid (9,76%), \u03b1-curcumene (7,07%), \u03b1-bisabolol (5,49%), Campesterol\r\n(4,41%), Stigmasterol (14,08%) and \u03b2-sitosterol (7,49%).\r\nConclusion: Our data suggest that bioactive compounds present in Ormenis eriolepis show significant anti proliferative\r\nactivity inducing cell cycle arrest and cell death operating through apoptosis pathway.<\/jats:p>","DOI":"10.21010\/ajtcam.v14i2.37","type":"journal-article","created":{"date-parts":[[2017,3,29]],"date-time":"2017-03-29T10:59:47Z","timestamp":1490785187000},"page":"356-373","source":"Crossref","is-referenced-by-count":18,"title":["INDUCTION OF CELL CYCLE ARREST AND APOPTOSIS BY ORMENIS ERIOLEPIS A MORROCAN ENDEMIC PLANT IN VARIOUS HUMAN CANCER CELL LINES"],"prefix":"10.21010","volume":"14","author":[{"given":"Lamiae","family":"Belayachi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clara","family":"Aceves-Luquero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nawel","family":"Merghoub","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia Fern\u00c3\u00a1ndez de","family":"Mattos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saa\u00c3\u00afd","family":"Amzazi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Priam","family":"Villalonga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Youssef","family":"Bakri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"8656","published-online":{"date-parts":[[2017,1,13]]},"container-title":["African Journal of Traditional, Complementary and Alternative Medicines"],"original-title":[],"link":[{"URL":"http:\/\/journals.athmsi.org\/index.php\/ajtcam\/article\/download\/4605\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.athmsi.org\/index.php\/ajtcam\/article\/download\/4605\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,8]],"date-time":"2023-05-08T08:42:40Z","timestamp":1683535360000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.athmsi.org\/index.php\/ajtcam\/article\/view\/4605"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,1,13]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2017,1,13]]}},"URL":"https:\/\/doi.org\/10.21010\/ajtcam.v14i2.37","relation":{},"ISSN":["2505-0044","0189-6016"],"issn-type":[{"value":"2505-0044","type":"electronic"},{"value":"0189-6016","type":"print"}],"subject":[],"published":{"date-parts":[[2017,1,13]]}}}