{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T09:17:40Z","timestamp":1762507060492},"reference-count":0,"publisher":"African Traditional Herbal Medicine Supporters Initiative (ATHMSI)","issue":"3","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Afr J  Tradit Complement Altern Med"],"abstract":"<jats:p>Background: Wedelia trilobata (L.) Hitch (WT), commonly known as yellow dots or creeping daisy, is a shrub&#x0D;\npossessing potent biological activities, and is traditionally used a medicinal plant in Ayurveda, Siddha and Unani&#x0D;\nsystems of medicines, and it has also been tried against leukemia cell line MEG- 01. In the present study, purification&#x0D;\nand screening of the plant was done for bioactive compounds in methanolic extract of WT for apoptotic and antileukemia&#x0D;\nactivity.&#x0D;\nMaterials and methods: The methanolic extract of WT was initially purified through thin layer chromatography&#x0D;\n(TLC) and screened for the apoptotic and anti-leukemia activities. The positive band of TLC was subjected to silica gel&#x0D;\ncolumn chromatography for further purification and the fractions obtained from it were screened again for antileukemia&#x0D;\nactivity through thymidine uptake assay and apoptotic activity by DNA fragmentation, nuclear staining and&#x0D;\nflow cytometry assays. The fraction with positive result was subjected to HPLC for analysis of bioactive components.&#x0D;\nResults: Out of many combinations of solvents, the methanol and dichloromethane combination in the ratio 6:4 has&#x0D;\nrevealed two bands in TLC, among which the second band showed positive results for apoptotic and anti-leukemic&#x0D;\nactivities. Further purification of second band through silica gel chromatography gave five fractions in which the 3rd&#x0D;\nfraction gave positive results and it shows single peak during compositional analysis through HPLC.&#x0D;\nConclusion: The single peak revealed through HPLC indicates the presence of pure compound with apoptotic and antileukemia&#x0D;\nactivities encouraging for further structural analysis.<\/jats:p>","DOI":"10.21010\/ajtcam.v14i3.18","type":"journal-article","created":{"date-parts":[[2017,3,29]],"date-time":"2017-03-29T13:00:04Z","timestamp":1490792404000},"page":"167-174","source":"Crossref","is-referenced-by-count":5,"title":["PURIFICATION AND FRACTIONAL ANALYSIS OF METHANOLIC EXTRACT OF WEDELIA TRILOBATA POSSESSING APOPTOTIC AND ANTI-LEUKEMIC ACTIVITY"],"prefix":"10.21010","volume":"14","author":[{"given":"Uday","family":"Venkatesh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chethan","family":"Javarasetty","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satish Kumar","family":"Murar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"8656","published-online":{"date-parts":[[2017,3,1]]},"container-title":["African Journal of Traditional, Complementary and Alternative Medicines"],"original-title":[],"link":[{"URL":"http:\/\/journals.athmsi.org\/index.php\/ajtcam\/article\/download\/4671\/pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/journals.athmsi.org\/index.php\/ajtcam\/article\/download\/4671\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,6]],"date-time":"2023-05-06T09:07:16Z","timestamp":1683364036000},"score":1,"resource":{"primary":{"URL":"http:\/\/journals.athmsi.org\/index.php\/ajtcam\/article\/view\/4671"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,3,1]]},"references-count":0,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2017,3,1]]}},"URL":"https:\/\/doi.org\/10.21010\/ajtcam.v14i3.18","relation":{},"ISSN":["2505-0044","0189-6016"],"issn-type":[{"value":"2505-0044","type":"electronic"},{"value":"0189-6016","type":"print"}],"subject":[],"published":{"date-parts":[[2017,3,1]]}}}