{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T23:39:10Z","timestamp":1788910750917,"version":"build-2803163510"},"reference-count":40,"publisher":"Korean Society of Otorhinolaryngology-Head and Neck Surgery","issue":"4","license":[{"start":{"date-parts":[[2020,4,13]],"date-time":"2020-04-13T00:00:00Z","timestamp":1586736000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100002471","name":"Dongguk University","doi-asserted-by":"publisher","award":["K-2016-G00002-00011"],"award-info":[{"award-number":["K-2016-G00002-00011"]}],"id":[{"id":"10.13039\/501100002471","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["e-ceo.org"],"crossmark-restriction":true},"short-container-title":["Clin Exp Otorhinolaryngol"],"published-print":{"date-parts":[[2020,11,1]]},"abstract":"<jats:p>Objectives. Human amniotic membrane extract (AME) is known to contain numerous bioactive factors and anti-inflammatory substances. However, the anti-inflammatory effects of AME on the middle ear (ME) mucosa are unclear. This study assessed the effects of AME on the growth of the ME mucosa in response to bacterially-induced otitis media (OM).Methods. OM was induced by inoculating nontypeable &lt;i&gt;Haemophilus influenzae&lt;\/i&gt; (NTHi) into the ME cavity of rats. ME mucosal explants were cultured in AME concentrations of 0, 5, 10, or 50 \u03bcg\/mL. The area of explant outgrowth was measured in culture and analyzed at 1, 3, 5, and 7 days after explantation. The expression of Ki-67, mucin 5AC (MUC5AC), tumor necrosis factor-\u03b1 (TNF-\u03b1), and interleukin-10 (IL-10) in the explants was also evaluated using quantitative polymerase chain reaction (PCR) and immunocytochemistry (ICC).Results. The NTHi-induced ME mucosa growth increased gradually over the 7-day culture period in all explants at different AME concentrations. There was a trend for mucosal growth inhibition at higher concentrations of AME, although the growth was not significantly different among the groups until day 5. The ME mucosal explants treated with the 50 \u03bcg\/mL concentration of AME showed significantly suppressed growth on postexplantation day 7 compared with other explants on the same day. PCR and ICC staining revealed that the expression of Ki-67, MUC5AC, TNF-\u03b1, and IL-10 further decreased in the explants with higher concentrations of AME than in those with lower concentrations of AME.Conclusion. Our results showed that higher concentrations of AME reduced the mucosal proliferative response in bacterial OM in rats. These findings provide evidence that AME has an influence on the inflammatory and proliferative responses to NTHi infection in ME mucosa.<\/jats:p>","DOI":"10.21053\/ceo.2019.01753","type":"journal-article","created":{"date-parts":[[2020,4,13]],"date-time":"2020-04-13T04:46:02Z","timestamp":1586753162000},"page":"381-388","update-policy":"https:\/\/doi.org\/10.21053\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Effects of Amniotic Membrane Extract on the Hyperplastic Response of the Middle Ear Mucosa in a Bacterially-Induced Otitis Media Rat Model: A Preliminary Study"],"prefix":"10.21053","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1499-3328","authenticated-orcid":true,"given":"Joo Hyun","family":"Park","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9084-9734","authenticated-orcid":true,"given":"Hee-Bok","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3558-6163","authenticated-orcid":true,"given":"Seo Hyun","family":"Ko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4645-0678","authenticated-orcid":true,"given":"Bo Hae","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5997-6193","authenticated-orcid":true,"given":"Yun-Sung","family":"Lim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4262-2150","authenticated-orcid":true,"given":"Seok-Won","family":"Park","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8488-9091","authenticated-orcid":true,"given":"Jae-Jun","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7103-8896","authenticated-orcid":true,"given":"Chang Gun","family":"Cho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1894","reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.3389\/fped.2013.00052"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)15495-0"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.epirev.a036129"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(00)00271-1"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1080\/14992020400050062"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.16.2.230-241.2003"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/162.3.685"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1177\/000348947108000611"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1159\/000057786"},{"key":"ref10","first-page":"356","volume-title":"","author":"Bluestone","year":"1983"},{"key":"ref11","volume-title":"Pathology of the ear","author":"Schuknecht"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1177\/000348948609500307"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1177\/1753425916668581"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1086\/593213"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1177\/1753425909103170"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2006.02.004"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1086\/345798"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.2174\/1574888X11308010003"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1007\/s10561-012-9337-x"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1097\/00003226-200005000-00018"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(80)91617-7"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1097\/01.TP.0000144606.84234.49"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1016\/0143-4004(91)90010-D"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-2115(00)00345-6"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.2010.184259"},{"key":"ref26","first-page":"177","volume-title":"New clinical application of amniotic membrane transplant for ocular surface disease","author":"Gheorghe","year":"2016"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.4103\/jispcd.JISPCD_359_16"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-012-1424-6"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-3768.2010.02097.x"},{"key":"ref30","first-page":"314","volume-title":"Amniotic membrane extract preparation: what is the best method","author":"Mahbod","year":"2014"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.01948"},{"key":"ref32","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijporl.2016.07.001"},{"key":"ref33","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijporl.2018.06.030"},{"key":"ref34","first-page":"e171","volume-title":"Promotion of osteogenic differentiation by amnion\/chorion membrane extracts","author":"Go","year":"2016"},{"key":"ref35","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0182716"},{"key":"ref36","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2007.05.006"},{"key":"ref37","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.08-1781"},{"key":"ref38","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2005.06.022"},{"key":"ref39","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(200003)182:3<311::AID-JCP1>3.0.CO;2-9"},{"key":"ref40","doi-asserted-by":"publisher","DOI":"10.1186\/1755-8794-5-21"}],"container-title":["Clinical and Experimental Otorhinolaryngology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/e-ceo.org\/upload\/pdf\/ceo-2019-01753.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/e-ceo.org\/journal\/view.php?doi=10.21053\/ceo.2019.01753","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/e-ceo.org\/upload\/pdf\/ceo-2019-01753.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,11,12]],"date-time":"2020-11-12T05:04:11Z","timestamp":1605157451000},"score":1,"resource":{"primary":{"URL":"http:\/\/e-ceo.org\/journal\/view.php?doi=10.21053\/ceo.2019.01753"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11,1]]},"references-count":40,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2020,11]]}},"URL":"https:\/\/doi.org\/10.21053\/ceo.2019.01753","relation":{},"ISSN":["1976-8710","2005-0720"],"issn-type":[{"value":"1976-8710","type":"print"},{"value":"2005-0720","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,11,1]]},"assertion":[{"value":"2019-10-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-11-26","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-04-13","order":2,"name":"published","label":"Published online","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-11-01","order":3,"name":"published","label":"Print publication","group":{"name":"publication_history","label":"Publication History"}}]}}