{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,13]],"date-time":"2026-11-13T10:21:13Z","timestamp":1794565273621,"version":"4.9.1"},"reference-count":47,"publisher":"Korean Society of Otorhinolaryngology-Head and Neck Surgery","issue":"2","license":[{"start":{"date-parts":[[2024,2,16]],"date-time":"2024-02-16T00:00:00Z","timestamp":1708041600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/"}],"funder":[{"name":"Suzhou Clinical Medical Center of Otorhinolaryngology, Head and Neck Surgery","award":["SZLCYXZX 202102"],"award-info":[{"award-number":["SZLCYXZX 202102"]}]},{"name":"Suzhou People\u2019s Livelihood Science and Technology Project","award":["SYS2020115"],"award-info":[{"award-number":["SYS2020115"]}]}],"content-domain":{"domain":["e-ceo.org"],"crossmark-restriction":true},"short-container-title":["Clin Exp Otorhinolaryngol"],"published-print":{"date-parts":[[2024,5,31]]},"abstract":"<jats:p>Objectives. The annual prevalence of chronic rhinosinusitis (CRS) is increasing, and the lack of effective treatments imposes a substantial burden on both patients and society. The formation of nasal polyps in patients with CRS is closely related to tissue remodeling, which is largely driven by the epithelial-mesenchymal transition (EMT). MicroRNA (miRNA) plays a pivotal role in the pathogenesis of numerous diseases through the miRNA-mRNA regulatory network; however, the specific mechanism of the miRNAs involved in the formation of nasal polyps remains unclear.Methods. The expression of EMT markers and Smad3 were detected using western blots, quantitative real-time polymerase chain reaction, and immunohistochemical and immunofluorescence staining. Differentially expressed genes in nasal polyps and normal tissues were screened through the Gene Expression Omnibus database. To predict the target genes of miR-145-5p, three different miRNA target prediction databases were used. The migratory ability of cells was evaluated using cell migration assay and wound healing assays.Results. miR-145-5p was associated with the EMT process and was significantly downregulated in nasal polyp tissues. In vitro experiments revealed that the downregulation of miR-145-5p promoted EMT. Conversely, increasing miR-145-5p levels reversed the EMT induced by transforming growth factor-\u03b21. Bioinformatics analysis suggested that miR-145-5p targets Smad3. Subsequent experiments confirmed that miR-145-5p inhibits Smad3 expression.Conclusion. Overall, miR-145-5p is a promising target to inhibit nasal polyp formation, and the findings of this study provide a theoretical basis for nanoparticle-mediated miR-145-5p delivery for the treatment of nasal polyps.<\/jats:p>","DOI":"10.21053\/ceo.2023.00025","type":"journal-article","created":{"date-parts":[[2024,2,16]],"date-time":"2024-02-16T04:19:00Z","timestamp":1708057140000},"page":"122-136","update-policy":"https:\/\/doi.org\/10.21053\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["MicroRNA-145-5p Regulates the Epithelial-Mesenchymal Transition in Nasal Polyps by Targeting Smad3"],"prefix":"10.21053","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-8044-3576","authenticated-orcid":true,"given":"Mengyu","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5472-9468","authenticated-orcid":true,"given":"Xiaole","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6551-9469","authenticated-orcid":true,"given":"Xiaolong","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-0021-7316","authenticated-orcid":true,"given":"Wentao","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-6302-8931","authenticated-orcid":true,"given":"Yuqing","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-1114-5688","authenticated-orcid":true,"given":"Fan","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-9931-8964","authenticated-orcid":true,"given":"Xiaomin","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4375-2803","authenticated-orcid":true,"given":"Dechun","family":"Geng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6694-9997","authenticated-orcid":true,"given":"Manyi","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1894","reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572-020-00218-1"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1016\/j.alit.2019.07.002"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2020.01.019"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1056\/nejmcp1800215"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaip.2022.01.016"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.4193\/rhin19.424"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-021-00442-x"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-018-0080-4"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23105848"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1002\/med.21596"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2020.114301"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.28830"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2021.05.020"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2021.04.024"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.12932\/ap-040921-1224"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21186878"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2017.08.034"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2022.153780"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19071901"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24032453"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.1002\/cac2.12138"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1038\/nrd.2016.246"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.47203"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2019.10.007"},{"key":"ref25","first-page":"10439","volume-title":"miR-145-5p inhibits psoriasis progression by regulating the Wnt\/\u03b2-catenin pathway","author":"Wang","year":"2021"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2019.11.040"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2021.643504"},{"key":"ref28","first-page":"1","volume-title":"European position paper on rhinosinusitis and nasal polyps 2020","author":"Fokkens","year":"2020"},{"key":"ref29","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.941608"},{"key":"ref30","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2022.02.016"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.1111\/all.15384"},{"key":"ref32","doi-asserted-by":"publisher","DOI":"10.1186\/s12929-020-00632-3"},{"key":"ref33","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab1256"},{"key":"ref34","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3758"},{"key":"ref35","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.14389"},{"key":"ref36","doi-asserted-by":"publisher","DOI":"10.1016\/j.burns.2023.09.009"},{"key":"ref37","doi-asserted-by":"publisher","DOI":"10.1016\/j.chest.2019.12.014"},{"key":"ref38","doi-asserted-by":"publisher","DOI":"10.1177\/19458924231152683"},{"key":"ref39","doi-asserted-by":"publisher","DOI":"10.1159\/000497829"},{"key":"ref40","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-020-01598-7"},{"key":"ref41","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18091833"},{"key":"ref42","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-022-10182-6"},{"key":"ref43","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2023.09.020"},{"key":"ref44","doi-asserted-by":"publisher","DOI":"10.1002\/alr.23076"},{"key":"ref45","doi-asserted-by":"publisher","DOI":"10.1111\/all.15796"},{"key":"ref46","doi-asserted-by":"publisher","DOI":"10.1038\/nmat3765"},{"key":"ref47","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2022.114113"}],"container-title":["Clinical and Experimental Otorhinolaryngology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/e-ceo.org\/upload\/pdf\/ceo-2023-00025.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/e-ceo.org\/journal\/view.php?doi=10.21053\/ceo.2023.00025","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/e-ceo.org\/upload\/pdf\/ceo-2023-00025.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T07:15:38Z","timestamp":1717139738000},"score":1,"resource":{"primary":{"URL":"http:\/\/e-ceo.org\/journal\/view.php?doi=10.21053\/ceo.2023.00025"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,31]]},"references-count":47,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2024,5]]}},"URL":"https:\/\/doi.org\/10.21053\/ceo.2023.00025","relation":{},"ISSN":["1976-8710","2005-0720"],"issn-type":[{"value":"1976-8710","type":"print"},{"value":"2005-0720","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,31]]},"assertion":[{"value":"2023-10-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-16","order":2,"name":"published","label":"Published online","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-31","order":3,"name":"published","label":"Print publication","group":{"name":"publication_history","label":"Publication History"}}]}}