{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T00:20:17Z","timestamp":1793751617030,"version":"4.6.1"},"reference-count":49,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"10","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,5,17]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>The high incidence of incomplete or failed healing after rotator cuff repair (RCR) has led to an increased focus on the biologic factors that affect tendon-to-bone healing. Inflammation plays a critical role in the initial tendon-healing response. C-C chemokine receptor type 2 (CCR2) is a chemokine receptor linked to the recruitment of monocytes in early inflammatory stages and is associated with an increase in pro-inflammatory macrophages. The purpose of this study was to evaluate the role of CCR2 in tendon healing following RCR in C57BL\/6J wildtype (WT) and CCR2\u2212\/\u2212 knockout (CCR2KO) mice in a delayed RCR model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>Fifty-two 12-week-old, male mice were allocated to 2 groups (WT and CCR2KO). All mice underwent unilateral supraspinatus tendon (SST) detachment at the initial surgical procedure, followed by a delayed repair 2 weeks later. The primary outcome measure was biomechanical testing. Secondary measures included histology, gene expression analysis, flow cytometry, and gait analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>The mean load-to-failure was 1.64 \u00b1 0.41 N in the WT group and 2.50 \u00b1 0.42 N in the CCR2KO group (p = 0.030). The mean stiffness was 1.43 \u00b1 0.66 N\/mm in the WT group and 3.00 \u00b1 0.95 N\/mm in the CCR2KO group (p = 0.008). Transcriptional profiling demonstrated 7 differentially expressed genes (DEGs) when comparing the CCR2KO and WT groups (p &lt; 0.05) and significant differences in Type-I and Type-II interferon pathway scores (p &lt; 0.01). Flow cytometry demonstrated significant differences between groups for the percentage of macrophages present (8.1% for the WT group compared with 5.8% for the CCR2KO group; p = 0.035). Gait analysis demonstrated no significant differences between groups.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>CCR2KO may potentially improve tendon biomechanical properties by decreasing macrophage infiltration and\/or by suppressing inflammatory mediator pathways in the setting of delayed RCR.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Clinical Relevance:<\/jats:title>\n                    <jats:p>CCR2 may be a promising target for novel therapeutics that aim to decrease failure rates following RCR.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.2106\/jbjs.22.01160","type":"journal-article","created":{"date-parts":[[2023,3,22]],"date-time":"2023-03-22T15:07:35Z","timestamp":1679497655000},"page":"779-788","update-policy":"https:\/\/doi.org\/10.1097\/lww.0000000000001000","source":"Crossref","is-referenced-by-count":20,"title":["Effect of CCR2 Knockout on Tendon Biomechanical Properties in a Mouse Model of Delayed Rotator Cuff Repair"],"prefix":"10.2106","volume":"105","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4298-5755","authenticated-orcid":false,"given":"Claire D.","family":"Eliasberg","sequence":"first","affiliation":[{"name":"Orthopaedic Soft Tissue Research Program, Hospital for Special Surgery, New York, NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3605-919X","authenticated-orcid":false,"given":"Camila B.","family":"Carballo","sequence":"additional","affiliation":[{"name":"Orthopaedic Soft Tissue Research Program, Hospital for Special 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