{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T17:18:36Z","timestamp":1788369516368,"version":"build-2803163510"},"reference-count":50,"publisher":"Ovid Technologies (Wolters Kluwer Health)","issue":"17","content-domain":{"domain":["lww.com","ovid.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2026,9,2]]},"abstract":"<jats:sec>\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>Limited vascularization at the tendon-bone interface (TBI) hinders rotator cuff (RC) healing. Although cell sheet technology has shown promise for interfacial repair, prevascularization strategies remain underexplored.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>Twenty female New Zealand rabbits underwent bilateral infraspinatus tendon repair and were randomized to receive either bone marrow-derived mesenchymal stem cell (BMSC) sheets or prevascularized BMSC sheets generated by coculture with endothelial cells, implanted at the TBI. An age- and weight-matched uninjured group served as a control. Healing at 6 weeks was assessed by gross observation, histology, immunohistochemistry, gene expression, and biomechanical testing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>\n                      Prevascularization of the BMSC sheets enhanced TBI vascularization, indicated by greater density of \u03b1-smooth muscle actin-positive vessels (16.16 \u00b1 2.81 versus 10.63 \u00b1 2.79\/mm\n                      <jats:sup>2<\/jats:sup>\n                      , p = 0.0079). Immunohistochemistry demonstrated greater areas positive for collagen type II alpha 1 (86.96 \u00b1 29.95 versus 40.25 \u00b1 11.96 \u03bcm\n                      <jats:sup>2<\/jats:sup>\n                      , p = 0.0079) and interleukin 10 (14.93 \u00b1 4.79 versus 7.43 \u00b1 2.48 \u03bcm\n                      <jats:sup>2<\/jats:sup>\n                      , p = 0.0159). Biomechanically, prevascularization of the sheets yielded greater ultimate failure load (156.89 \u00b1 51.92 versus 111.67 \u00b1 27.51 N, p = 0.0364) and stiffness (37.27 \u00b1 12.16 versus 27.16 \u00b1 7.33 N\/mm, p = 0.0486).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>Prevascularization of BMSC sheets was able to promote angiogenesis and improve structural and mechanical aspects of tendon-bone healing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Clinical Relevance:<\/jats:title>\n                    <jats:p>Prevascularized BMSC sheets may represent a biologic adjunct to enhance tendon-bone healing in RC repair.<\/jats:p>\n                  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The Second Xiangya Hospital, Central South University, Changsha, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5728-7036","authenticated-orcid":false,"given":"Yaxi","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology, School of Basic Medicine Science, Central South University, Changsha, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3465-3857","authenticated-orcid":false,"given":"Ding","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, The Second Xiangya Hospital, Central South University, Changsha, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7420-3319","authenticated-orcid":false,"given":"Lele","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, The Second Xiangya Hospital, Central South University, Changsha, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-2363-692X","authenticated-orcid":false,"given":"Wanyun","family":"Li","sequence":"additional","affiliation":[{"name":"School of Clinical Medicine, Guilin Medical University, Guilin, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6906-5350","authenticated-orcid":false,"given":"Qian","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics, The Second Xiangya Hospital, Central South University, Changsha, People\u2019s Republic of 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