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(Annelida), and Xenoturbella japonica (Xenacoelomorpha).\" e59161 (2019)","DOI":"10.3791\/59161"},{"key":"11","doi-asserted-by":"crossref","unstructured":"T Ikenaga, R Shimomai, H Hagio, et al.  \"J Comp Neurol.\"  530, \"Morphological analysis of the cerebellum and its efferent system in a basal actinopterygian fish, Polypterus senegalus.\" 1231 (2022)","DOI":"10.1002\/cne.25271"},{"key":"1","doi-asserted-by":"crossref","unstructured":"F Babaei, TL Hong, K Yeung, et al.  \"Zebrafish.\"  13, \"Contrast-enhanced X-ray micro-computed tomography as a versatile method for anatomical studies of adult zebrafish.\" 310 (2016)","DOI":"10.1089\/zeb.2016.1245"},{"key":"2","doi-asserted-by":"crossref","unstructured":"U Bickmeyer, I Meinen, S Meyer, et al.  \"Mar Environ Res.\"  161, \"Fluorescence measurements of the marine flatworm Macrostomum lignano during exposure to TNT and its derivatives 2-ADNT and 4-ADNT.\" 105041 (2020)","DOI":"10.1016\/j.marenvres.2020.105041"},{"key":"3","doi-asserted-by":"crossref","unstructured":"J Bischof, ME Day, KA Miller, et al.  \"Dev Biol.\"  467, \"Nervous system and tissue polarity dynamically adapt to new morphologies in planaria.\" 51 (2020)","DOI":"10.1016\/j.ydbio.2020.08.009"},{"key":"4","doi-asserted-by":"crossref","unstructured":"V Camilieri-Asch, JA Shaw, KE Yopak, et al.  \"Brain Struct Funct.\"  225, \"Volumetric analysis and morphological assessment of the ascending olfactory pathway in an elasmobranch and a teleost using diceCT.\" 2347 (2020)","DOI":"10.1007\/s00429-020-02127-1"},{"key":"5","doi-asserted-by":"crossref","unstructured":"F Carbayo  and JW Lenihan  \"GigaScience.\"  5, \"Micro-computed tomography scan and virtual histological slide data for the land planarian Obama otavioi (Platyhelminthes).\" 13 (2016)","DOI":"10.1186\/s13742-016-0119-4"},{"key":"6","doi-asserted-by":"crossref","unstructured":"S Faulwetter, A Vasileiadou, M Kouratoras, et al.  \"Zookeys.\"  263, \"Micro-computed tomography: Introducing new dimensions to taxonomy.\" 1 (2013)","DOI":"10.3897\/zookeys.263.4261"},{"key":"7","doi-asserted-by":"crossref","unstructured":"KJ Hayworth, JL Morgan, R Schalek, et al.  \"Front Neural Circuits.\"  8, \"Imaging ATUM ultrathin section libraries with WaferMapper: a multi-scale approach to EM reconstruction of neural circuits.\" 68 (2014)","DOI":"10.3389\/fncir.2014.00068"},{"key":"8","doi-asserted-by":"crossref","unstructured":"P Heger, W Zheng, A Rottmann, et al.  \"eLife.\"  9, \"The genetic factors of bilaterian evolution.\" (2020)","DOI":"10.7554\/eLife.45530"},{"key":"9","doi-asserted-by":"crossref","unstructured":"P Henny, MT Brown, BR Micklem, et al.  \"Brain Struct Funct.\"  219, \"Stereological and ultrastructural quantification of the afferent synaptome of individual neurons.\" 631 (2014)","DOI":"10.1007\/s00429-013-0523-9"},{"key":"10","doi-asserted-by":"crossref","unstructured":"N Higuchi, K Kohno, T Kadowaki,  \"BMC Biol.\"  7, \"Specific retention of the protostome-specific PsGEF may parallel with the evolution of mushroom bodies in insect and lophotrochozoan brains.\" 21 (2009)","DOI":"10.1186\/1741-7007-7-21"},{"key":"12","doi-asserted-by":"crossref","unstructured":"CL Keenan, R Coss, H Koopowitz,  \"J Comp Neurol.\"  195, \"Cytoarchitecture of primitive brains: Golgi studies in flatworms.\" 697 (1981)","DOI":"10.1002\/cne.901950412"},{"key":"13","doi-asserted-by":"crossref","unstructured":"A Kobayashi, M Hamada, MA Yoshida, et al.  \"Sci Adv.\"  8, \"Vasopressin-oxytocin-type signaling is ancient and has a conserved water homeostasis role in euryhaline marine planarians.\" eabk0331 (2022)","DOI":"10.1126\/sciadv.abk0331"},{"key":"14","doi-asserted-by":"crossref","unstructured":"D Le, Z Sabry, A Chandra, et al.  \"Curr Biol.\"  31, \"Planarian fragments behave as whole animals.\" 5111 (2021)","DOI":"10.1016\/j.cub.2021.09.056"},{"key":"16","doi-asserted-by":"crossref","unstructured":"JM Martin-Duran  and A Hejnol  \"Dev Biol.\"  475, \"A developmental perspective on the evolution of the nervous system.\" 181 (2021)","DOI":"10.1016\/j.ydbio.2019.10.003"},{"key":"17","doi-asserted-by":"crossref","unstructured":"BD Metscher,  \"Dev Dyn.\"  238, \"MicroCT for developmental biology: a versatile tool for high-contrast 3D imaging at histological resolutions.\" 632 (2009)","DOI":"10.1002\/dvdy.21857"},{"key":"18","doi-asserted-by":"crossref","unstructured":"R Mizutani  and Y Suzuki  \"Micron.\"  43, \"X-ray microtomography in biology.\" 104 (2012)","DOI":"10.1016\/j.micron.2011.10.002"},{"key":"19","doi-asserted-by":"crossref","unstructured":"R Mizutani, A Takeuchi, T Hara, et al.  \"J Synchrotron Radiat.\"  14, \"Computed tomography imaging of the neuronal structure of Drosophila brain.\" 282 (2007)","DOI":"10.1107\/S0909049507009004"},{"key":"20","doi-asserted-by":"crossref","unstructured":"R Mizutani, R Saiga, A Takeuchi, et al.  \"J Struct Biol.\"  184, \"Three-dimensional network of Drosophila brain hemisphere.\" 271 (2013)","DOI":"10.1016\/j.jsb.2013.08.012"},{"key":"21","doi-asserted-by":"crossref","unstructured":"R Mizutani, R Saiga, S Takekoshi, et al.  \"Microscopy Today.\"  23, \"Scanning brain networks with micro-CT.\" 12 (2015)","DOI":"10.1017\/S1551929515000784"},{"key":"22","doi-asserted-by":"crossref","unstructured":"R Mizutani, R Saiga, M Ohtsuka, et al.  \"Sci Rep.\"  6, \"Three-dimensional X-ray visualization of axonal tracts in mouse brain hemisphere.\" 35061 (2016)","DOI":"10.1038\/srep35061"},{"key":"23","doi-asserted-by":"crossref","unstructured":"F Pradillon, A Schmidt, J Peplies, et al.  \"Mar Ecol Prog Ser.\"  333, \"Species identification of marine invertebrate early stages by whole-larvae in situ hybridisation of 18S ribosomal RNA.\" 103 (2007)","DOI":"10.3354\/meps333103"},{"key":"24","doi-asserted-by":"crossref","unstructured":"C Nore\u00f1a, C Damborenea, F Brusa,  \"Ecology and general biology. 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The pattern of FMRF-amide immunoreactivity in Acoela (Plathelminthes).\" 57 (1998)","DOI":"10.1016\/S0040-8166(98)80006-2"},{"key":"27","doi-asserted-by":"crossref","unstructured":"FA Rivera-Quiroz  and JA Miller  \"J Comp Neurol.\"  530, \"Micro-CT visualization of the CNS: Performance of different contrast-enhancing techniques for documenting the spider brain.\" 2474 (2022)","DOI":"10.1002\/cne.25343"},{"key":"28","doi-asserted-by":"crossref","unstructured":"Y Sakurai  and Y Ikeda  \"Microsc Res Tech.\"  82, \"Development of a contrast-enhanced micro computed tomography protocol for the oval squid (Sepioteuthis lessoniana) brain.\" 1941 (2019)","DOI":"10.1002\/jemt.23363"},{"key":"29","doi-asserted-by":"crossref","unstructured":"T Schadt, V Prantl, AL Grosbusch, et al.  \"Cell Tissue Res.\"  383, \"Regeneration of the flatworm Prosthiostomum siphunculus (Polycladida, Platyhelminthes).\" 1025 (2021)","DOI":"10.1007\/s00441-020-03302-w"},{"key":"30","doi-asserted-by":"crossref","unstructured":"MS Silva  and F Carbayo  \"ZooKeys.\"  910, \"X-ray microcomputed tomography applied to the taxonomic study of rare material: redescriptions of seven of Schirch's Brazilian species of land planarians (Geoplanidae, Platyhelminthes).\" 1 (2020)","DOI":"10.3897\/zookeys.910.39486.figure1"},{"key":"31","doi-asserted-by":"crossref","unstructured":"POM Steinhoff, A Sombke, J Liedtke, et al.  \"Arthropod Struct Dev.\"  46, \"The synganglion of the jumping spider Marpissa muscosa (Arachnida: Salticidae): Insights from histology, immunohistochemistry and microCT analysis.\" 156","DOI":"10.1016\/j.asd.2016.11.003"},{"key":"32","doi-asserted-by":"crossref","unstructured":"NJ Strausfeld,  \"J Comp Neurol.\"  450, \"Organization of the honey bee mushroom body: representation of the calyx within the vertical and gamma lobes.\" 4 (2002)","DOI":"10.1002\/cne.10285"},{"key":"33","doi-asserted-by":"crossref","unstructured":"NJ Strausfeld  and F Hirth  \"Philos Trans R Soc Lond B Biol Sci.\"  371, \"Introduction to \u2018Homology and convergence in nervous system evolution\" 20150034 (2016)","DOI":"10.1098\/rstb.2015.0034"},{"key":"34","doi-asserted-by":"crossref","unstructured":"S Udagawa, K Miyara, H Takekata, et al.  \"J Neurosci Methods.\"  328, \"Investigation on the validity of 3D micro-CT imaging in the fish brain.\" 108416 (2019)","DOI":"10.1016\/j.jneumeth.2019.108416"},{"key":"35","doi-asserted-by":"crossref","unstructured":"GH Wolff  and NJ Strausfeld  \"Curr Biol.\"  25, \"Genealogical correspondence of mushroom bodies across invertebrate phyla.\" 38 (2015)","DOI":"10.1016\/j.cub.2014.10.049"},{"key":"36","doi-asserted-by":"crossref","unstructured":"GH Wolff  and NJ Strausfeld  \"Philos Trans R Soc Lond B Biol Sci.\"  371, \"Genealogical correspondence of a forebrain centre implies an executive brain in the protostome-deuterostome bilaterian ancestor.\" 20150055 (2016)","DOI":"10.1098\/rstb.2015.0055"},{"key":"37","doi-asserted-by":"crossref","unstructured":"H Yamamoto  and K Agata  \"Dev Growth Differ.\"  53, \"Optic chiasm formation in planarian I: Cooperative netrin- and robo-mediated signals are required for the early stage of optic chiasm formation.\" 300 (2011)","DOI":"10.1111\/j.1440-169X.2010.01234.x"}],"container-title":["Zoological Science"],"original-title":[],"deposited":{"date-parts":[[2024,5,27]],"date-time":"2024-05-27T13:35:08Z","timestamp":1716816908000},"score":1,"resource":{"primary":{"URL":"https:\/\/bioone.org\/journals\/zoological-science\/volume-41\/issue-3\/zs230082\/Volume-X-Ray-Micro-Computed-Tomography-Analysis-of-the-Early\/10.2108\/zs230082.full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,4,8]]},"references-count":37,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2024,6,1]]}},"URL":"https:\/\/doi.org\/10.2108\/zs230082","relation":{},"ISSN":["0289-0003"],"issn-type":[{"value":"0289-0003","type":"print"}],"subject":[],"published":{"date-parts":[[2024,4,8]]}}}